BMRB Entry 6371

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6371

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Title:
1H, 13C, and 15N resonance assignments of Sin3a associated polypeptide 18 (SAP-18)
Deposition date:
2004-10-28
Original release date:
2005-04-08
Authors:
McCallum, Scott; Fairbrother, Wayne
Citation:

Citation: McCallum, Scott; Yin, JianPing; Fairbrother, Wayne. "Letters to the Editor: 1H, 13C, and 15N resonance assignments of SAP18"  J. Biomol. NMR 31, 259-259 (2005).

Assembly members:

Assembly members:
Sin3a associated polypeptide p18, polymer, 148 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET-28d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sin3a associated polypeptide p18: GSHMRVTQEEIKKEPEKPID REKTSPLLLRVFTTNNGRHH RMDEFSRGNVPSSELQIYTW MDATLKELTSLVKEVYPEAR KKGTHFNFAIVFTDVKRPGY RVKEIGSTMSGRKGTDDSMT LQSQKFQIGDYLDIAITPPN RAPPPSGR

Data sets:
Data typeCount
1H chemical shifts992
13C chemical shifts559
15N chemical shifts143

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SAP-18 monomer1

Entities:

Entity 1, SAP-18 monomer 148 residues - Formula weight is not available

1   GLYSERHISMETARGVALTHRGLNGLUGLU
2   ILELYSLYSGLUPROGLULYSPROILEASP
3   ARGGLULYSTHRSERPROLEULEULEUARG
4   VALPHETHRTHRASNASNGLYARGHISHIS
5   ARGMETASPGLUPHESERARGGLYASNVAL
6   PROSERSERGLULEUGLNILETYRTHRTRP
7   METASPALATHRLEULYSGLULEUTHRSER
8   LEUVALLYSGLUVALTYRPROGLUALAARG
9   LYSLYSGLYTHRHISPHEASNPHEALAILE
10   VALPHETHRASPVALLYSARGPROGLYTYR
11   ARGVALLYSGLUILEGLYSERTHRMETSER
12   GLYARGLYSGLYTHRASPASPSERMETTHR
13   LEUGLNSERGLNLYSPHEGLNILEGLYASP
14   TYRLEUASPILEALAILETHRPROPROASN
15   ARGALAPROPROPROSERGLYARG

Samples:

sample_1: Sin3a associated polypeptide p18, [U-99% 13C; U-99% 15N], 1 mM

Ex-cond_1: pH: 5.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
1H-13C NOESYnot availablenot availablenot available
1H-13C TOCSYnot availablenot availablenot available
1H-13C COSYnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HN(CO)CACBnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available

Software:

FELIX -

CYANA -

CNX -

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

PDB
DBJ BAB23871 BAB24687 BAB61044 BAC39588 BAE21751
EMBL CAB09797 CAG33316 CAH90072
GB AAC51322 AAD41090 AAF21220 AAH06625 AAH30836
REF NP_001028857 NP_001030544 NP_001129009 NP_001135913 NP_001232121
SP O00422 O55128 Q3T022 Q5RDT5
TPG DAA23863
AlphaFold O55128 O00422 Q3T022 Q5RDT5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks