BMRB Entry 6361

Title:
Resonance Assignments for the SAP domain of the human protein DEK
Deposition date:
2004-10-19
Original release date:
2005-02-10
Authors:
Devany, Matthew; Kotharu, N.; Matsuo, Hiroshi
Citation:

Citation: Devany, Matthew; Matsuo, Hiroshi. "Letter to the Editor: NMR resonance assignments for the DNA-supercoiling domain of the human protein DEK"  J. Biomol. NMR 31, 65-65 (2005).

Assembly members:

Assembly members:
DEK, polymer, 109 residues, 12688 Da.

Natural source:

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts707
13C chemical shifts447
15N chemical shifts112

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DEK78-1861

Entities:

Entity 1, DEK78-186 109 residues - 12688 Da.

1   PHETHRILEALAGLNGLYLYSGLYGLNLYS
2   LEUCYSGLUILEGLUARGILEHISPHEPHE
3   LEUSERLYSLYSLYSTHRASPGLULEUARG
4   ASNLEUHISLYSLEULEUTYRASNARGPRO
5   GLYTHRVALSERSERLEULYSLYSASNVAL
6   GLYGLNPHESERGLYPHEPROPHEGLULYS
7   GLYSERVALGLNTYRLYSLYSLYSGLUGLU
8   METLEULYSLYSPHEARGASNALAMETLEU
9   LYSSERILECYSGLUVALLEUASPLEUGLU
10   ARGSERGLYVALASNSERGLULEUVALLYS
11   ARGILELEUASNPHELEUMETHISPRO

Samples:

sample_1: DEK, [U-13C; U-15N], 0.7 mM; Na2HPO4 25 mM; NaH2PO4 25 mM; KCl 100 mM; DTT 20 mM; pefabloc 0.4 mM; NaN3 0.02%; DTT 20 mM

cond-1: pH: 6.8; temperature: 298 K; ionic strength: 0.15 M

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCOnot availablenot availablenot available
CBCANHnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
HCCHTOCSYnot availablenot availablenot available
1H-13C NOESYHSQCnot availablenot availablenot available
1H-15N NOESYHSQCnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAB25100 BAG35503 BAG60099 BAG60375 BAG72522
EMBL CAA45536
GB AAH35259 AAH79344 AAI42199 ABZ92195 ADP20523
REF NP_001004255 NP_001093187 NP_001128181 NP_001244457 NP_001266767
SP P35659 Q6AXS3
TPG DAA16149
AlphaFold Q6AXS3 P35659

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks