BMRB Entry 6224

Name: Assignment of the 1H, 15N and 13C resonances of SufA from E. coli
Published: 2004-12-16

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6224

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assignment of the 1H, 15N and 13C resonances of SufA from E. coli

Deposition date:

2004-06-04

Original release date:

2004-12-16

Authors:

Duraffourg, Nicolas; Ollagnier-de-Choudens, Sandrine; Fontecave, Marc; Loiseau, Laurent; Barras, Frederic; Marion, Dominique; Blanchard, Laurence

Citation:

Citation: Duraffourg, Nicolas; Ollagnier-de-Choudens, Sandrine; Fontecave, Marc; Loiseau, Laurent; Barras, Frederic; Marion, Dominique; Blanchard, Laurence. "Letter to the Editor: Assignment of the 1H, 15N and 13C resonances of SufA from Escherichia coli involved in Fe-S cluster biosynthesis" J. Biomol. NMR 30, 379-380 (2004).

Assembly members:

Assembly members:
SufA, polymer, 130 residues, 14480 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SufA: MDMHSGTFNPQDFAWQGLTL TPAAAIHIRELVAKQPGMVG VRLGVKQTGCAGFGYVLDSV SEPDKDDLLFEHDGAKLFVP LQAMPFIDGTEVDFVREGLN QIFKFHNPKAQNECGCGESF GVLEHHHHHH

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	566
13C chemical shifts	433
15N chemical shifts	120

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SufA	1

Entities:

Entity 1, SufA 130 residues - 14480 Da.

1	MET	ASP	MET	HIS	SER	GLY	THR	PHE	ASN	PRO
2	GLN	ASP	PHE	ALA	TRP	GLN	GLY	LEU	THR	LEU
3	THR	PRO	ALA	ALA	ALA	ILE	HIS	ILE	ARG	GLU
4	LEU	VAL	ALA	LYS	GLN	PRO	GLY	MET	VAL	GLY
5	VAL	ARG	LEU	GLY	VAL	LYS	GLN	THR	GLY	CYS
6	ALA	GLY	PHE	GLY	TYR	VAL	LEU	ASP	SER	VAL
7	SER	GLU	PRO	ASP	LYS	ASP	ASP	LEU	LEU	PHE
8	GLU	HIS	ASP	GLY	ALA	LYS	LEU	PHE	VAL	PRO
9	LEU	GLN	ALA	MET	PRO	PHE	ILE	ASP	GLY	THR
10	GLU	VAL	ASP	PHE	VAL	ARG	GLU	GLY	LEU	ASN
11	GLN	ILE	PHE	LYS	PHE	HIS	ASN	PRO	LYS	ALA
12	GLN	ASN	GLU	CYS	GLY	CYS	GLY	GLU	SER	PHE
13	GLY	VAL	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

Samples:

sample_1: SufA, [U-15N; U-13C], 1.0 mM

cond_1: pH: 7; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	not available	not available	not available
3D HNCA	not available	not available	not available
3D CBCA(CO)NH	not available	not available	not available
3D CBCANH	not available	not available	not available
3D HNCACB	not available	not available	not available
3D HNCO	not available	not available	not available
3D HN(CA)CO	not available	not available	not available
3D HC(C)H-TOCSY	not available	not available	not available
3D (H)CC(CO)NH-TOCSY	not available	not available	not available
3D H(CC)(CO)NH-TOCSY	not available	not available	not available

Software:

FELIX v2000.1 -

NMR spectrometers:

Varian INOVA 600 MHz

PDB	2D2A
DBJ	BAA15453 BAB35814 BAG77331 BAI25659 BAI30634
EMBL	CAP76181 CAQ98593 CAR03043 CAR08076 CAR13169
GB	AAC74754 AAG56671 AAN43290 AAN80539 AAP17178
REF	NP_288118 NP_310418 NP_416199 NP_707583 NP_753974
SP	P77667
AlphaFold	P77667

1	MET	ASP	MET	HIS	SER	GLY	THR	PHE	ASN	PRO
2	GLN	ASP	PHE	ALA	TRP	GLN	GLY	LEU	THR	LEU
3	THR	PRO	ALA	ALA	ALA	ILE	HIS	ILE	ARG	GLU
4	LEU	VAL	ALA	LYS	GLN	PRO	GLY	MET	VAL	GLY
5	VAL	ARG	LEU	GLY	VAL	LYS	GLN	THR	GLY	CYS
6	ALA	GLY	PHE	GLY	TYR	VAL	LEU	ASP	SER	VAL
7	SER	GLU	PRO	ASP	LYS	ASP	ASP	LEU	LEU	PHE
8	GLU	HIS	ASP	GLY	ALA	LYS	LEU	PHE	VAL	PRO
9	LEU	GLN	ALA	MET	PRO	PHE	ILE	ASP	GLY	THR
10	GLU	VAL	ASP	PHE	VAL	ARG	GLU	GLY	LEU	ASN
11	GLN	ILE	PHE	LYS	PHE	HIS	ASN	PRO	LYS	ALA
12	GLN	ASN	GLU	CYS	GLY	CYS	GLY	GLU	SER	PHE
13	GLY	VAL	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	MET	ASP	MET	HIS	SER	GLY	THR	PHE	ASN	PRO
2	GLN	ASP	PHE	ALA	TRP	GLN	GLY	LEU	THR	LEU
3	THR	PRO	ALA	ALA	ALA	ILE	HIS	ILE	ARG	GLU
4	LEU	VAL	ALA	LYS	GLN	PRO	GLY	MET	VAL	GLY
5	VAL	ARG	LEU	GLY	VAL	LYS	GLN	THR	GLY	CYS
6	ALA	GLY	PHE	GLY	TYR	VAL	LEU	ASP	SER	VAL
7	SER	GLU	PRO	ASP	LYS	ASP	ASP	LEU	LEU	PHE
8	GLU	HIS	ASP	GLY	ALA	LYS	LEU	PHE	VAL	PRO
9	LEU	GLN	ALA	MET	PRO	PHE	ILE	ASP	GLY	THR
10	GLU	VAL	ASP	PHE	VAL	ARG	GLU	GLY	LEU	ASN
11	GLN	ILE	PHE	LYS	PHE	HIS	ASN	PRO	LYS	ALA
12	GLN	ASN	GLU	CYS	GLY	CYS	GLY	GLU	SER	PHE
13	GLY	VAL	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS