BMRB Entry 6223

Title:
Sequential resonance assignment of yeast cytosine deaminase in complex with a transition state analogue
Deposition date:
2004-06-02
Original release date:
2007-03-22
Authors:
Yao, Lishan; Liu, Aizhuo; Li, Yue; Felczak, Krzysztof; Li, Guangyu; Yan, Honggao
Citation:

Citation: Liu, Aizhuo; Li, Yue; Yao, L.; Yan, Honggao. "Simultaneous NMR assignment of backbone and side chain amides in large proteins with IS-TROSY"  J. Biomol. NMR 36, 205-214 (2006).
PubMed: 17091334

Assembly members:

Assembly members:
Yeast cytosine deaminase, polymer, 158 residues, Formula weight is not available
5-fluorine, 2-hydroxypyrimidine, non-polymer, 114.0778232 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts308
13C chemical shifts424
15N chemical shifts145

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YCD monomer 11
2YCD monomer 21
35-fluorine, 2-hydroxypyrimidine2
4ZINC (II) ION3

Entities:

Entity 1, YCD monomer 1 158 residues - Formula weight is not available

1   METVALTHRGLYGLYMETALASERLYSTRP
2   ASPGLNLYSGLYMETASPILEALATYRGLU
3   GLUALAALALEUGLYTYRLYSGLUGLYGLY
4   VALPROILEGLYGLYCYSLEUILEASNASN
5   LYSASPGLYSERVALLEUGLYARGGLYHIS
6   ASNMETARGPHEGLNLYSGLYSERALATHR
7   LEUHISGLYGLUILESERTHRLEUGLUASN
8   CYSGLYARGLEUGLUGLYLYSVALTYRLYS
9   ASPTHRTHRLEUTYRTHRTHRLEUSERPRO
10   CYSASPMETCYSTHRGLYALAILEILEMET
11   TYRGLYILEPROARGCYSVALVALGLYGLU
12   ASNVALASNPHELYSSERLYSGLYGLULYS
13   TYRLEUGLNTHRARGGLYHISGLUVALVAL
14   VALVALASPASPGLUARGCYSLYSLYSILE
15   METLYSGLNPHEILEASPGLUARGPROGLN
16   ASPTRPPHEGLUASPILEGLYGLU

Entity 2, 5-fluorine, 2-hydroxypyrimidine 1 residues - C4 H3 N2 O F - 114.0778232 Da.

1   PYR1

Entity 3, ZINC (II) ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Yeast cytosine deaminase, [U-70% 2H, U-100% 13C, U-100% 15N], 1.8 mM

Ex-cond_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_1not availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

REF NP_015387
SWISS-PROT Q12178
GenBank EDN61192 EDV11264 AAC13409 AAG33626 AAB67713
EMBL CAA95006 CAA89179
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks