BMRB Entry 6125

Title:
1H, 13C, 15N chemical shift assignments of an enolase-phosphatase E1 from Klebsiella oxytoca
Deposition date:
2004-03-03
Original release date:
2004-12-16
Authors:
Kostic, Milka; Pochapsky, Thomas
Citation:

Citation: Kostic, Milka; Pochapsky, Thomas. "1H, 13C and 15N chemical shift assignments of an enolase-phosphatase, E1, from Klebsiella oxytoca"  J. Biomol. NMR 30, 359-360 (2004).

Assembly members:

Assembly members:
enolase-phosphatase, polymer, 228 residues, 25613.8 Da.
MG, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: Klebsiella oxytoca   Taxonomy ID: 571   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Klebsiella oxytoca

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pTCP01

Data sets:
Data typeCount
13C chemical shifts859
15N chemical shifts228
1H chemical shifts1248

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1E1 monomer1
2Mg2+2

Entities:

Entity 1, E1 monomer 228 residues - 25613.8 Da.

1   ILEARGALAILEVALTHRASPILEGLUGLY
2   THRTHRSERASPILEARGPHEVALHISASN
3   VALLEUPHEPROTYRALAARGGLUARGLEU
4   ALAGLYPHEVALTHRALAGLNGLNPHEVAL
5   GLUPROVALLYSTHRILELEUASPASNLEU
6   ARGGLUGLUILEALAGLNPROALAALAGLY
7   ALAGLUGLULEUILEALATHRLEUPHEALA
8   PHEMETASPGLUASPARGLYSSERTHRALA
9   LEULYSALALEUGLNGLYILEILETRPARG
10   ASPGLYTYRVALHISGLYASPPHETHRGLY
11   HISLEUTYRPROASPVALLEUPROALALEU
12   GLULYSTRPLYSSERGLNGLYILEASPLEU
13   TYRVALTYRSERSERGLYSERVALALAALA
14   GLNLYSLEULEUPHEGLYTYRSERASPGLU
15   GLYASPILETHRHISLEUPHEASNGLYTYR
16   PHEASPTHRLEUVALGLYALALYSARGGLU
17   ALAGLNSERTYRARGASNILEALAGLUGLN
18   LEUGLYGLNPROPROALAALAILELEUPHE
19   LEUSERASPILEHISGLNGLULEUASPALA
20   ALAGLUGLUALAGLYPHEARGTHRLEUGLN
21   LEUVALARGGLYASPARGASPPROALASER
22   HISHISPROGLNVALGLNARGPHEASPASP
23   ILEHISPROGLUGLNILEPROALA

Entity 2, Mg2+ - Mg - 24.305 Da.

1   MG

Samples:

sample_1: enolase-phosphatase, [U-13C; U-N15], 0.5 – 1 mM

sample_2: enolase-phosphatase, [U-15N], 1.0 mM

sample_3: enolase-phosphatase, [15N]-Thr, 1.0 mM

Ex-cond_1: pH: 7.4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availableEx-cond_1
HN(CO)CAnot availablenot availableEx-cond_1
HNCACBnot availablenot availableEx-cond_1
CBCA(CO)NHnot availablenot availableEx-cond_1
HCCONHnot availablenot availableEx-cond_1
1H-15N HSQCnot availablenot availableEx-cond_1
1H-15N NOESYnot availablenot availableEx-cond_1
1H-15N TOCSYnot availablenot availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

GB AAC43183 AFN31361 EHT02105 EHT02397 EHT03381
REF WP_004100131 WP_004130394 WP_004848743 WP_014837800 WP_024358822
SP Q48389
AlphaFold Q48389

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks