BMRB Entry 6031

Title:
1H, 13C, and 15N resonance assignments of human Notch-1 calcium binding EGF domains 11-13
Deposition date:
2003-12-03
Original release date:
2004-07-06
Authors:
Muranyi, Andreas; Hambleton, Sophie; Knott, Vroni; McMichael, Andrew; Handford, Penny; Downing, A. Kristina
Citation:

Citation: Muranyi, Andreas; Hambleton, Sophie; Knott, Vroni; McMichael, Andrew; Handford, Penny; Downing, A.. "Letter to the Editor: 1H, 13C, and 15N resonance assignments of human Notch-1 calcium binding EGF domains 11-13"  J. Biomol. NMR 29, 443-444 (2004).
PubMed: 15213460

Assembly members:

Assembly members:
human Notch-1, polymer, 118 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo Sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts382
15N chemical shifts118
1H chemical shifts661

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hN11-131
2Calcium ion2
3Calcium ion2
4Calcium ion2

Entities:

Entity 1, hN11-13 118 residues - Formula weight is not available

1   SERALAGLNASPVALASPGLUCYSSERLEU
2   GLYALAASNPROCYSGLUHISALAGLYLYS
3   CYSILEASNTHRLEUGLYSERPHEGLUCYS
4   GLNCYSLEUGLNGLYTYRTHRGLYPROARG
5   CYSGLUILEASPVALASNGLUCYSVALSER
6   ASNPROCYSGLNASNASPALATHRCYSLEU
7   ASPGLNILEGLYGLUPHEGLNCYSILECYS
8   METPROGLYTYRGLUGLYVALHISCYSGLU
9   VALASNTHRASPGLUCYSALASERSERPRO
10   CYSLEUHISASNGLYARGCYSLEUASPLYS
11   ILEASNGLUPHEGLNCYSGLUCYSPROTHR
12   GLYPHETHRGLYHISLEUCYSGLN

Entity 2, Calcium ion - Ca - 40.078 Da.

1   CA

Samples:

15N_sample: human Notch-1, [U-15N], 1.0 mM; CaCl2 15 mM; NaN3 0.02%

13C_15N_sample: human Notch-1, [U-13C; U-15N], 0.5 mM

Ex-cond_1: ionic strength: 0.09 M; pH: 6.1; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N TOCSY-HSQCnot availablenot availableEx-cond_1
1H-15N NOESY-HSQCnot availablenot availableEx-cond_1
HNCAnot availablenot availableEx-cond_1
HN(C0)CAnot availablenot availableEx-cond_1
HNCACBnot availablenot availableEx-cond_1
CBCA(CO)NHnot availablenot availableEx-cond_1
HNCOnot availablenot availableEx-cond_1
HCCH-TOCSYnot availablenot availableEx-cond_1
1H-13C NOESYnot availablenot availableEx-cond_1

Software:

FELIX v2.3 - Processing

NMRView v5.0 - Analysis

formatconverter v1.0 b2 - Format conversion to NMRStar

NMR spectrometers:

  • GE Omega 600 MHz
  • GE Omega 750 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks