BMRB Entry 6021

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6021

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Title:
Backbone 1H, 15N and 13C assignments for the subunit a of the E.coli ATP Synthase
Deposition date:
2003-11-24
Original release date:
2004-07-06
Authors:
Dmitriev, Oleg; Abildgaard, Frits; Markley, John; Fillingame, Robert
Citation:

Citation: Dmitriev, Oleg; Abildgaard, Frits; Markley, John; Fillingame, Robert. "Letter to the Editor: Backbone 1H, 15N and 13C assignments for the subunit a of the E.coli ATP Synthase "  J. Biomol. NMR 29, 439-440 (2004).
PubMed: 15213458

Assembly members:

Assembly members:
subunit a of the ATP synthase, polymer, 277 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pBWU13

Entity Sequences (FASTA):

Entity Sequences (FASTA):
subunit a of the ATP synthase: MHHHHHHASENMTPQDYIGH HLNNLQLDLRTFSLVDPQNP PATFWTINIDSMFFSVVLGL LFLVLFRSVAKKATSGVPGK FQTAIELVIGFVNGSVKDMY HGKSKLIAPLALTIFVWVFL MNLMDLLPIDLLPYIAEHVL GLPALRVVPSADVNVTLSMA LGVFILILFYSIKMKGIGGF TKELTLQPFNHWAFIPVNLI LEGVSLLSKPVSLGLRLFGN MYAGELIFILIAGLLPWWSQ WILNVPWAIFHILIITLQAF IFMVLTIVYLSMASEEH

Data sets:
Data typeCount
1H chemical shifts244
13C chemical shifts753
15N chemical shifts244

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1sibunit a1

Entities:

Entity 1, sibunit a 277 residues - Formula weight is not available

1   METHISHISHISHISHISHISALASERGLU
2   ASNMETTHRPROGLNASPTYRILEGLYHIS
3   HISLEUASNASNLEUGLNLEUASPLEUARG
4   THRPHESERLEUVALASPPROGLNASNPRO
5   PROALATHRPHETRPTHRILEASNILEASP
6   SERMETPHEPHESERVALVALLEUGLYLEU
7   LEUPHELEUVALLEUPHEARGSERVALALA
8   LYSLYSALATHRSERGLYVALPROGLYLYS
9   PHEGLNTHRALAILEGLULEUVALILEGLY
10   PHEVALASNGLYSERVALLYSASPMETTYR
11   HISGLYLYSSERLYSLEUILEALAPROLEU
12   ALALEUTHRILEPHEVALTRPVALPHELEU
13   METASNLEUMETASPLEULEUPROILEASP
14   LEULEUPROTYRILEALAGLUHISVALLEU
15   GLYLEUPROALALEUARGVALVALPROSER
16   ALAASPVALASNVALTHRLEUSERMETALA
17   LEUGLYVALPHEILELEUILELEUPHETYR
18   SERILELYSMETLYSGLYILEGLYGLYPHE
19   THRLYSGLULEUTHRLEUGLNPROPHEASN
20   HISTRPALAPHEILEPROVALASNLEUILE
21   LEUGLUGLYVALSERLEULEUSERLYSPRO
22   VALSERLEUGLYLEUARGLEUPHEGLYASN
23   METTYRALAGLYGLULEUILEPHEILELEU
24   ILEALAGLYLEULEUPROTRPTRPSERGLN
25   TRPILELEUASNVALPROTRPALAILEPHE
26   HISILELEUILEILETHRLEUGLNALAPHE
27   ILEPHEMETVALLEUTHRILEVALTYRLEU
28   SERMETALASERGLUGLUHIS

Samples:

sample_1: subunit a of the ATP synthase, [U-13C; U-15N; non-exchangeable 90% 2H], 0.3 mM; CD3COONa 25 mM; CHCl3:CD3OH:H2O (4:4:1) 100%

conditions: pH*: 5.5; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
HNCOnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CACBnot availablenot availablenot available
HN(CA)CBnot availablenot availablenot available
HNCOCAnot availablenot availablenot available
HNCACOnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 750 MHz

Related Database Links:

PDB
DBJ BAB38103 BAE77550 BAG79552 BAI27998 BAI33121
EMBL CAA23514 CAA23520 CAA23521 CAA23590 CAA25776
GB AAA24731 AAA24740 AAA62090 AAA83869 AAC76761
REF NP_290377 NP_312707 NP_418194 NP_709551 NP_756524
SP A1AHR8 A7ZTV0 A8A6K1 B1IX00 B1LL65
AlphaFold A1AHR8 A7ZTV0 A8A6K1 B1IX00 B1LL65

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks