BMRB Entry 5853

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5853

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Sequential 1H, 15N and 13C NMR assignment of human Calbindin D28k
Deposition date:
2003-06-30
Original release date:
2004-02-11
Authors:
Helgstrand, Magnus; Vanbelle, Christophe; Thulin, Eva; Linse, Sara; Akke, Mikael
Citation:

Citation: Helgstrand, Magnus; Vanbelle, Christophe; Thulin, Eva; Linse, Sara; Akke, Mikael. "Letter to the Editor: Sequential 1H, 15N and 13C NMR assignment of human Calbindin D28k"  J. Biomol. NMR 28, 305-306 (2004).
PubMed: 14752267

Assembly members:

Assembly members:
Calbindin D28k, polymer, 261 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Calbindin D28k: MEASHLQSSLITASQFFEIW LHFDADGSGYLEGKELQNLI QELQQARKKAGLELSPEMKT FVDQYGQRDDGKIGIVELAH VLPTEENFLLLFRSQQLKSS EEFMKTWRKYDTDHSGFIET EELKNFLKDLLEKANKTVDD TKLAEYTDLMLKLFDSNNDG KLELTEMARLLPVQENFLLK FEGIKMSGKEFNKAFELYDQ DGDGYIDENELDALLKDLSE KNKQDLDINNITTYKKNIMA LSDGGKLYRTDLALILSAGD N

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts706
15N chemical shifts240
1H chemical shifts240

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Calbindin D28k1
2CALCIUM (II) ION, 12
3CALCIUM (II) ION, 22
4CALCIUM (II) ION, 32
5CALCIUM (II) ION, 42

Entities:

Entity 1, Calbindin D28k 261 residues - Formula weight is not available

1   METGLUALASERHISLEUGLNSERSERLEU
2   ILETHRALASERGLNPHEPHEGLUILETRP
3   LEUHISPHEASPALAASPGLYSERGLYTYR
4   LEUGLUGLYLYSGLULEUGLNASNLEUILE
5   GLNGLULEUGLNGLNALAARGLYSLYSALA
6   GLYLEUGLULEUSERPROGLUMETLYSTHR
7   PHEVALASPGLNTYRGLYGLNARGASPASP
8   GLYLYSILEGLYILEVALGLULEUALAHIS
9   VALLEUPROTHRGLUGLUASNPHELEULEU
10   LEUPHEARGSERGLNGLNLEULYSSERSER
11   GLUGLUPHEMETLYSTHRTRPARGLYSTYR
12   ASPTHRASPHISSERGLYPHEILEGLUTHR
13   GLUGLULEULYSASNPHELEULYSASPLEU
14   LEUGLULYSALAASNLYSTHRVALASPASP
15   THRLYSLEUALAGLUTYRTHRASPLEUMET
16   LEULYSLEUPHEASPSERASNASNASPGLY
17   LYSLEUGLULEUTHRGLUMETALAARGLEU
18   LEUPROVALGLNGLUASNPHELEULEULYS
19   PHEGLUGLYILELYSMETSERGLYLYSGLU
20   PHEASNLYSALAPHEGLULEUTYRASPGLN
21   ASPGLYASPGLYTYRILEASPGLUASNGLU
22   LEUASPALALEULEULYSASPLEUSERGLU
23   LYSASNLYSGLNASPLEUASPILEASNASN
24   ILETHRTHRTYRLYSLYSASNILEMETALA
25   LEUSERASPGLYGLYLYSLEUTYRARGTHR
26   ASPLEUALALEUILELEUSERALAGLYASP
27   ASN

Entity 2, CALCIUM (II) ION, 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: Calbindin D28k, [U-13C; U-15N], 1.0 mM; CaCl2 11.0 mM; Mes-buffer 20.0 mM; EDTA 1.0 mM; NaN3 0.02%

sample_2: Calbindin D28k, [U-15N], 2.0 mM; Mes-buffer 20.0 mM; CaCl2 11.0 mM; NaN3 0.02%

Exp_condition_1: pH: 6.5; temperature: 323 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availableExp_condition_1
HNCAnot availablenot availableExp_condition_1
HNCACBnot availablenot availableExp_condition_1
CBCA(CO)NHnot availablenot availableExp_condition_1
HNCOnot availablenot availableExp_condition_1
(HA)CO(CA)NHnot availablenot availableExp_condition_1
C(CO)NH-TOCSYnot availablenot availableExp_condition_1
H(CCO)NH-TOCSYnot availablenot availableExp_condition_1
3D 1H-15N NOESYnot availablenot availableExp_condition_1

Software:

NMRPipe - data processing

Ansig for Windows - assignment

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 800 MHz

Related Database Links:

REF XP_004679800

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks