BMRB Entry 5835

Title:
The Solution Structure and Dynamics of Recombinant Onconases
Deposition date:
2003-06-17
Original release date:
2004-03-07
Authors:
Gorbatyuk, Vitaliy; Tsai, Chen-Kuon; Chang, Chi-Fon; Huang, Tai-huang
Citation:

Citation: Gorbatyuk, Vitaliy; Tsai, Chen-Kuon; Chang, Chi-Fon; Huang, Tai-huang. "Effect of N-terminal and Met23 mutations on the structure and dynamics of onconase"  J. Biol. Chem. 279, 5772-5780 (2004).
PubMed: 14645226

Assembly members:

Assembly members:
(M-1,Q1,M23L)rONC, polymer, 105 residues, 11958 Da.

Natural source:

Natural source:   Common Name: Northern leopard frog   Taxonomy ID: 8404   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rana pipiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-11d

Data sets:
Data typeCount
coupling constants84

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1(M-1,Q1,M23L)rOnc1

Entities:

Entity 1, (M-1,Q1,M23L)rOnc 105 residues - 11958 Da.

1   METGLNASPTRPLEUTHRPHEGLNLYSLYS
2   HISILETHRASNTHRARGASPVALASPCYS
3   ASPASNILELEUSERTHRASNLEUPHEHIS
4   CYSLYSASPLYSASNTHRPHEILETYRSER
5   ARGPROGLUPROVALLYSALAILECYSLYS
6   GLYILEILEALASERLYSASNVALLEUTHR
7   THRSERGLUPHETYRLEUSERASPCYSASN
8   VALTHRSERARGPROCYSLYSTYRLYSLEU
9   LYSLYSSERTHRASNLYSPHECYSVALTHR
10   CYSGLUASNGLNALAPROVALHISPHEVAL
11   GLYVALGLYSERCYS

Samples:

sample_1: (M-1,Q1,M23L)rONC, [U-15N; U-13C], 1.5 mM

sample_conditions_set_1: pH*: 4.1; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
3D HNHAsample_1not availablesample_conditions_set_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 4371
PDB
GenBank AAL54383
SWISS-PROT P22069