BMRB Entry 5803

Title:
Three-disulfide variant of hen lysozyme: C64A/C80A
Deposition date:
2003-05-19
Original release date:
2004-09-14
Authors:
Yokota, Atsushi; Hirai, Kenichi; Miyauchi, Hiroyo; Noda, Yasuo; Inoue, Kyouko; Akasaka, Kazuyuki; Tachibana, Hideki; Segawa, Shin-ichi
Citation:

Citation: Yokota, Atsushi; Hirai, Kenichi; Miyauchi, Hiroyo; Iimura, Satoshi; Noda, Yasuo; Inoue, Kyouko; Akasaka, Kazuyuki; Tachibana, Hideki; Segawa, Shin-ichi. "NMR characterization of three-disulfide variants of lysozyme, C64A/C80A, C76A/C94A, and C30A/C115A--a marginally stable state in folded proteins."  Biochemistry 43, 6663-6669 (2004).
PubMed: 15157100

Assembly members:

Assembly members:
three-disulfide variant of hen lysozyme, polymer, 130 residues, 14374 Da.

Natural source:

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts680

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hen lysozyme Cys mutants, C64A/C80A1

Entities:

Entity 1, hen lysozyme Cys mutants, C64A/C80A 130 residues - 14374 Da.

1   METLYSVALPHEGLYARGCYSGLULEUALA
2   ALAALAMETLYSARGHISGLYLEUASPASN
3   TYRARGGLYTYRSERLEUGLYASNTRPVAL
4   CYSALAALALYSPHEGLUSERASNPHEASN
5   THRGLNALATHRASNARGASNTHRASPGLY
6   SERTHRASPTYRGLYILELEUGLNILEASN
7   SERARGTRPTRPALAASNASPGLYARGTHR
8   PROGLYSERARGASNLEUCYSASNILEPRO
9   ALASERALALEULEUSERSERASPILETHR
10   ALASERVALASNCYSALALYSLYSILEVAL
11   SERASPGLYASNGLYMETASNALATRPVAL
12   ALATRPARGASNARGCYSLYSGLYTHRASP
13   VALGLNALATRPILEARGGLYCYSARGLEU

Samples:

sample_1: three-disulfide variant of hen lysozyme, [U-15N], 0.3 – 1.0 mM

EX-cond_1: pH: 3.8 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
HOHAHAnot availablenot availablenot available
15N 3D-NOESY-HSQCnot availablenot availablenot available
15N 3D-TOCSY-HSQCnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available
15N 3D-HSQC-NOESY-HSQCnot availablenot availablenot available

Software:

XWINNMR v2.6 -

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

SWISS-PROT P00698
GenBank AAL69327 AAA48943
PDB
BMRB 5069 5068 4943 4562 1093