BMRB Entry 5799

Title:
Protein Signal Assignments using Specific Labeling and Cell-free Synthesis
Deposition date:
2003-05-16
Original release date:
2004-03-23
Authors:
Shi, Jianxia; Pelton, Jefferey
Citation:

Citation: Shi, Jianxia; Pelton, Jefferey; Cho, H.; Wemmer, D.. "Protein Signal Assignments using Specific Labeling and Cell-free Synthesis"  J. Biomol. NMR 28, 235-247 (2004).
PubMed: 14752257

Assembly members:

Assembly members:
Phosphoserine phosphatase, polymer, 211 residues, 23594 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 2190   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Methanococcous jannichi

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts557
13C chemical shifts599
15N chemical shifts206

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PSP monomer1

Entities:

Entity 1, PSP monomer 211 residues - 23594 Da.

1   METGLULYSLYSLYSLYSLEUILELEUPHE
2   ASPPHEASPSERTHRLEUVALASNASNGLU
3   THRILEASPGLUILEALAARGGLUALAGLY
4   VALGLUGLUGLUVALLYSLYSILETHRLYS
5   GLUALAMETGLUGLYLYSLEUASNPHEGLU
6   GLNSERLEUARGLYSARGVALSERLEULEU
7   LYSASPLEUPROILEGLULYSVALGLULYS
8   ALAILELYSARGILETHRPROTHRGLUGLY
9   ALAGLUGLUTHRILELYSGLULEULYSASN
10   ARGGLYTYRVALVALALAVALVALSERGLY
11   GLYPHEASPILEALAVALASNLYSILELYS
12   GLULYSLEUGLYLEUASPTYRALAPHEALA
13   ASNARGLEUILEVALLYSASPGLYLYSLEU
14   THRGLYASPVALGLUGLYGLUVALLEULYS
15   GLUASNALALYSGLYGLUILELEUGLULYS
16   ILEALALYSILEGLUGLYILEASNLEUGLU
17   ASPTHRVALALAVALGLYASPGLYALAASN
18   ASPILESERMETPHELYSLYSALAGLYLEU
19   LYSILEALAPHECYSALALYSPROILELEU
20   LYSGLULYSALAASPILECYSILEGLULYS
21   ARGASPLEUARGGLUILELEULYSTYRILE
22   LYS

Samples:

Sample1: Phosphoserine phosphatase 1.0 mM

Ex-cond_1: pH: 6.2 na; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
15N hsqcnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNcaCOnot availablenot availablenot available
HNcoCAnot availablenot availablenot available
CBCAcoNHnot availablenot availablenot available
HAHNnot availablenot availablenot available
HBHAcoNHnot availablenot availablenot available
HNcaNHnot availablenot availablenot available
15N NOESYnot availablenot availablenot available

Software:

NMRPipe -

NMRView -

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
GB AAB99612 AIJ05608
REF NP_248603 WP_010871118
SP Q58989
AlphaFold Q58989

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks