BMRB Entry 5789

Title:
1H,15N, and 13C chemical shift assignments of the Escherichia coli nitrogen regulatory phosphocarrier IIANtr
Deposition date:
2003-04-29
Original release date:
2004-06-25
Authors:
Li, Xia; Peterkofsky, Alan; Wang, Guangshun
Citation:

Citation: Li, Xia; Peterkofsky, Alan; Wang, Guangshun. "Letter to the Editor: 1H,15N, and 13C Chemical Shift Assignments of the Escherichia coli Nitrogen Regulatory Phosphocarrier IIA(Ntr)"  J. Biomol. NMR 27, 401-402 (2003).
PubMed: 14512741

Assembly members:

Assembly members:
nitrogen regulatory enzyme IIA, polymer, 163 residues, 18000 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRE-His-Tag

Data sets:
Data typeCount
1H chemical shifts924
13C chemical shifts653
15N chemical shifts161

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IIANtr1

Entities:

Entity 1, IIANtr 163 residues - 18000 Da.

1   METTHRASNASNASPTHRTHRLEUGLNLEU
2   SERSERVALLEUASNARGGLUCYSTHRARG
3   SERARGVALHISCYSGLNSERLYSLYSARG
4   ALALEUGLUILEILESERGLULEUALAALA
5   LYSGLNLEUSERLEUPROPROGLNVALVAL
6   PHEGLUALAILELEUTHRARGGLULYSMET
7   GLYSERTHRGLYILEGLYASNGLYILEALA
8   ILEPROHISGLYLYSLEUGLUGLUASPTHR
9   LEUARGALAVALGLYVALPHEVALGLNLEU
10   GLUTHRPROILEALAPHEASPALAILEASP
11   ASNGLNPROVALASPLEULEUPHEALALEU
12   LEUVALPROALAASPGLNTHRLYSTHRHIS
13   LEUHISTHRLEUSERLEUVALALALYSARG
14   LEUALAASPLYSTHRILECYSARGARGLEU
15   ARGALAALAGLNSERASPGLUGLULEUTYR
16   GLNILEILETHRASPTHRGLUGLYTHRPRO
17   ASPGLUALA

Samples:

sample_1: nitrogen regulatory enzyme IIA, [U-95% 13C; U-95% 15N], 1.0 mM

sample_cond_1: pH: 7.3; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCAnot availablenot availablenot available
CA(CO)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
H(CCO)NHnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available

Software:

NMRPipe, PIPP - data processing, peak picking

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA02317 BAB37506 BAE77248 BAG79012 BAI27482
EMBL CAA81619 CAP77664 CAQ33537 CAR00166 CAR04814
GB AAA58006 AAB60165 AAC76236 AAG58338 AAN44710
REF NP_289778 NP_312110 NP_417671 NP_709003 NP_755830
SP P69815 P69829
AlphaFold P69815 P69829

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks