BMRB Entry 5615

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5615

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H/13C/15N assignment of the C-terminal domain of the E. amylovora response regulator RcsB

Deposition date:

2002-12-04

Original release date:

2003-12-11

Authors:

Pristovsek, Primoz; SenGupta, Kaushik; Loehr, Frank; SenGupta, Soma; Bernhard, Frank; Rueterjans, Heinz

Citation:

Citation: Pristovsek, Primoz; SenGupta, Kaushik; Loehr, Frank; Schafer, B.; Trebra, M.; Rueterjans, Heinz; Bernhard, Frank. "Structural analysis of the DNA-binding domain of the Erwinia amylovora RcsB protein and its interaction with the RcsAB box" J. Biol. Chem. 278, 17752-17759 (2003).
PubMed: 12740396

Assembly members:

Assembly members:
response regulator, polymer, 99 residues, 10989 Da.

Natural source:

Natural source: Common Name: Erwinia amylovora Taxonomy ID: 552 Superkingdom: Eubacteria Kingdom: not available Genus/species: Erwinia Amylovora

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
response regulator: MRGSHHHHHHGSYTPESVAK LLEKISAGGYGDKRLSPKES EVLRLFAEGFLVTEIAKKLN RSIKTISSQKKSAMMKLGVD NDIALLNYLSSVSMTPVDK

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	632
13C chemical shifts	375
15N chemical shifts	92

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RcsB monomer	1

Entities:

Entity 1, RcsB monomer 99 residues - 10989 Da.

1

MET

ARG

GLY

SER

HIS

2

GLY

SER

TYR

THR

PRO

GLU

SER

VAL

ALA

LYS

3

LEU

GLU

LYS

ILE

SER

ALA

GLY

TYR

4

GLY

ASP

LYS

ARG

LEU

SER

PRO

LYS

GLU

SER

5

GLU

VAL

LEU

ARG

LEU

PHE

ALA

GLU

GLY

PHE

6

LEU

VAL

THR

GLU

ILE

ALA

LYS

LEU

ASN

7

ARG

SER

ILE

LYS

THR

ILE

SER

GLN

LYS

8

LYS

SER

ALA

MET

LYS

LEU

GLY

VAL

ASP

9

ASN

ASP

ILE

ALA

LEU

ASN

TYR

LEU

SER

10

SER

VAL

SER

MET

THR

PRO

VAL

ASP

LYS

Samples:

sample_1: response regulator, [U-99% 13C; U-99% 15N], 0.8 – 1.2 mM

cond_1: pH: 6.4; temperature: 289 K

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

FELIX v97.0 - processing, visualisation

st2nmr v1.05 - semi-automated sequence-specific assignment

NMR spectrometers:

Bruker DRX 600 MHz
Bruker DRX 800 MHz
Bruker DMX 500 MHz

PDB	1P4W
DBJ	BAN97122 GAJ89526
EMBL	CAA70978 CAO96276 CAX55067 CAY73751 CBA21538
GB	ADP13087 ADU69998 AIX73097 EKV53357 EOS96240
REF	WP_004158545 WP_004172989 WP_012440971 WP_012667617 WP_013509853