BMRB Entry 5601

Title:
Complete 1H,13C,15N assignments of zinc Pyrococcus furiosus rubredoxin
Deposition date:
2002-11-26
Original release date:
2003-05-14
Authors:
Bougault, Catherine; Eidsness, Marly; Prestegard, James
Citation:

Citation: Bougault, Catherine; Eidsness, Marly; Prestegard, James. "Hydrogen Bonds in Rubredoxins from Mesophilic and Hyperthermophilic Organisms"  Biochemistry 42, 4357-4372 (2003).
PubMed: 12693931

Assembly members:

Assembly members:
pyrococcus furiosus rubredoxin, polymer, 54 residues, 6443.68 Da.
pyrococcus furiosus rubredoxin, polymer, 54 residues, 6414.67 Da.
pyrococcus furiosus rubredoxin, polymer, 53 residues, 6277.52 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Pyrococcus furiosus   Taxonomy ID: 2261   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrococcus furiosus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pEt T7

Entity Sequences (FASTA):

Entity Sequences (FASTA):
pyrococcus furiosus rubredoxin: XAKWVCKICGYIYDEDAGDP DNGISPGTKFEELPDDWVCP ICGAPKSEFEKLED
pyrococcus furiosus rubredoxin: MAKWVCKICGYIYDEDAGDP DNGISPGTKFEELPDDWVCP ICGAPKSEFEKLED
pyrococcus furiosus rubredoxin: AKWVCKICGYIYDEDAGDPD NGISPGTKFEELPDDWVCPI CGAPKSEFEKLED

Data typeCount
1H chemical shifts476
15N chemical shifts159
13C chemical shifts550
coupling constants108

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1fMetPfRdZn1
2Zn14
3MetPfRdZn2
4Zn24
5WT-PfRdZn3
6Zn34

Entities:

Entity 1, fMetPfRdZn 54 residues - 6443.68 Da.

1   FMEALALYSTRPVALCYSLYSILECYSGLY
2   TYRILETYRASPGLUASPALAGLYASPPRO
3   ASPASNGLYILESERPROGLYTHRLYSPHE
4   GLUGLULEUPROASPASPTRPVALCYSPRO
5   ILECYSGLYALAPROLYSSERGLUPHEGLU
6   LYSLEUGLUASP

Entity 4, Zn1 - Zn - 65.409 Da.

1   ZN

Entity 2, MetPfRdZn 54 residues - 6414.67 Da.

1   METALALYSTRPVALCYSLYSILECYSGLY
2   TYRILETYRASPGLUASPALAGLYASPPRO
3   ASPASNGLYILESERPROGLYTHRLYSPHE
4   GLUGLULEUPROASPASPTRPVALCYSPRO
5   ILECYSGLYALAPROLYSSERGLUPHEGLU
6   LYSLEUGLUASP

Entity 3, WT-PfRdZn 53 residues - 6277.52 Da.

1   ALALYSTRPVALCYSLYSILECYSGLYTYR
2   ILETYRASPGLUASPALAGLYASPPROASP
3   ASNGLYILESERPROGLYTHRLYSPHEGLU
4   GLULEUPROASPASPTRPVALCYSPROILE
5   CYSGLYALAPROLYSSERGLUPHEGLULYS
6   LEUGLUASP

Samples:

sample_1: pyrococcus furiosus rubredoxin, [U-100% 13C; U-100% 15N], 3.29 mM; pyrococcus furiosus rubredoxin, [U-100% 13C; U-100% 15N], 0.65 mM; pyrococcus furiosus rubredoxin, [U-100% 13C; U-100% 15N], 0.65 mM

Condition_1: pH: 6.44 na; temperature: 298 K; ionic strength: 0.29 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availableCondition_1
1H-13C HSQCsample_1not availableCondition_1
HNCOsample_1not availableCondition_1
HNCACBsample_1not availableCondition_1
HCACOsample_1not availableCondition_1
1H-13C HSQC-TOCSYsample_1not availableCondition_1
1H-13C HSQC-NOESYsample_1not availableCondition_1
(HB)CB(CGCD)HDsample_1not availableCondition_1
(HB)CB(CGCDCE)HEsample_1not availableCondition_1
HCCH-TOCSYsample_1not availableCondition_1
HCCO2sample_1not availableCondition_1

Software:

FELIX v2000 -

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

SWISS-PROT P24297 P24297 P24297
REF NP_579011.1 NP_579011.1 NP_579011.1
GenBank AAL81406.1 AAF03228.1 AAB20233.1 AAL81406.1 AAF03228.1 AAB20233.1 AAL81406.1 AAF03228.1 AAB20233.1
PDB
BMRB 5601 5601 1991 5601 5601 1991 5601 5601 1991

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks