BMRB Entry 5559

Title:
Partial 1H and 13C assignments for 3'-stem-loop of human U4 small nuclear RNA
Deposition date:
2002-10-15
Original release date:
2002-12-27
Authors:
Comolli, L.; Ulyanov, N.; James, T.; Gmeiner, W.
Citation:

Citation: Comolli, L.; Ulyanov, N.; Soto, A.; Marky, L.; James, T.; Gmeiner, W.. "NMR Structure of the 3' Stem-Loop from Human U4 snRNA"  Nucleic Acids Res. 30, 4371-4379 (2002).

Assembly members:

Assembly members:
single chain of RNA, polymer, 20 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: enzymatic semisynthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
single chain of RNA: GACAGUCUCUACGGAGACUG

Data sets:
Data typeCount
13C chemical shifts67
1H chemical shifts117

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
13'SL of U4 snRNA1

Entities:

Entity 1, 3'SL of U4 snRNA 20 residues - Formula weight is not available

1   GACAGUCUCU
2   ACGGAGACUG

Samples:

sample_1: single chain of RNA 1 mM; sodium phosphate 10 mM; NaCl 50 mM

sample_cond_1: ionic strength: 60 mM; pH: 6.4; pressure: 1 atm; temperature: 303 K

sample_cond_2: ionic strength: 60 mM; pH: 6.4; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
2D SS-NOESYnot availablenot availablenot available
2D DQF-COSYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
natural abundance 13C-HMQCnot availablenot availablenot available

Software:

VNMR v6.1 - collection

NMRPipe v2.1 - processing

SPARKY v3.0 - data analysis

MARDIGRAS v3.2 - iterative matrix relaxation

DYANA v1.5 - refinement

miniCarlo vn/a - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz