BMRB Entry 5474

Title:
Backbone resonance assignment of the 298 amino acid catalytic domain of protein tyrosine phosphatase 1B (PTP1B)
Deposition date:
2002-07-24
Original release date:
2003-02-03
Authors:
Meier, Sebastian; Li, Yu-Chin; Koehn, James; Vlattas, Isidoros; Wareing, James; Jahnke, Wolfgang; Wennogle, Lawrence; Grzesiek, Stephan
Citation:

Citation: Meier, Sebastian; Li, Yu-Chin; Koehn, James; Vlattas, Isidoros; Wareing, James; Jahnke, Wolfgang; Wennogle, Lawrence; Grzesiek, Stephan. "Letter to the Editor: Backbone resonance assignment of the 298 amino acid catalytic domain of protein tyrosine phosphatase 1B (PTP1B)"  J. Biomol. NMR 24, 165-166 (2002).

Assembly members:

Assembly members:
protein tyrosine phosphatase 1B, polymer, 298 residues, 34672 Da.
DADEXLIP-amide,(X=phenylalanine-p-difluoromethyl phosphonate), polymer, 8 residues, 34672 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 19-b

Data sets:
Data typeCount
1H chemical shifts218
13C chemical shifts697
15N chemical shifts218

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein tyrosine phosphatase 1B1
2DADEXLIP-amide,(X=phenylalanine-p-difluoromethyl phosphonate)2

Entities:

Entity 1, protein tyrosine phosphatase 1B 298 residues - 34672 Da.

1   METGLUMETGLULYSGLUPHEGLUGLNILE
2   ASPLYSSERGLYSERTRPALAALAILETYR
3   GLNASPILEARGHISGLUALASERASPPHE
4   PROCYSARGVALALALYSLEUPROLYSASN
5   LYSASNARGASNARGTYRARGASPVALSER
6   PROPHEASPHISSERARGILELYSLEUHIS
7   GLNGLUASPASNASPTYRILEASNALASER
8   LEUILELYSMETGLUGLUALAGLNARGSER
9   TYRILELEUTHRGLNGLYPROLEUPROASN
10   THRCYSGLYHISPHETRPGLUMETVALTRP
11   GLUGLNLYSSERARGGLYVALVALMETLEU
12   ASNARGVALMETGLULYSGLYSERLEULYS
13   CYSALAGLNTYRTRPPROGLNLYSGLUGLU
14   LYSGLUMETILEPHEGLUASPTHRASNLEU
15   LYSLEUTHRLEUILESERGLUASPILELYS
16   SERTYRTYRTHRVALARGGLNLEUGLULEU
17   GLUASNLEUTHRTHRGLNGLUTHRARGGLU
18   ILELEUHISPHEHISTYRTHRTHRTRPPRO
19   ASPPHEGLYVALPROGLUSERPROALASER
20   PHELEUASNPHELEUPHELYSVALARGGLU
21   SERGLYSERLEUSERPROGLUHISGLYPRO
22   VALVALVALHISCYSSERALAGLYILEGLY
23   ARGSERGLYTHRPHECYSLEUALAASPTHR
24   CYSLEULEULEUMETASPLYSARGLYSASP
25   PROSERSERVALASPILELYSLYSVALLEU
26   LEUGLUMETARGLYSPHEARGMETGLYLEU
27   ILEGLNTHRALAASPGLNLEUARGPHESER
28   TYRLEUALAVALILEGLUGLYALALYSPHE
29   ILEMETGLYASPSERSERVALGLNASPGLN
30   TRPLYSGLULEUSERHISGLUASP

Entity 2, DADEXLIP-amide,(X=phenylalanine-p-difluoromethyl phosphonate) 8 residues - 34672 Da.

1   ASPALAASPGLUXLEUILEPRO

Samples:

sample_1: protein tyrosine phosphatase 1B, [U-100% 15N; U-100% 13C], 0.5 mM; DADEXLIP 1 mM; H2O 30%; D2O 70%

Ex-cond_1: pH: 7.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
HN(CO)CAsample_1not availableEx-cond_1
HN(CA)COsample_1not availableEx-cond_1
HN(CO)CACBsample_1not availableEx-cond_1
HNCACBsample_1not availableEx-cond_1
HNCAsample_1not availableEx-cond_1
HNCOsample_1not availableEx-cond_1
HN(CO)CA TROSYsample_1not availableEx-cond_1
HN(CA)CO TROSYsample_1not availableEx-cond_1
HN(CO)CACB TROSYsample_1not availableEx-cond_1
HNCACB TROSYsample_1not availableEx-cond_1
HNCA TROSYsample_1not availableEx-cond_1
HNCO TROSYsample_1not availableEx-cond_1
15N-edited NOESYsample_1not availableEx-cond_1

Software:

nmrPIPE -

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

SWISS-PROT P18031
REF XP_001096412 XP_001096290 XP_001096168 XP_001096053 NP_002818
GenBank AAP35398 AAH18164 AAH15660 AAA60223 AAA60157
EMBL CAI23215 CAH90487 CAC00618
DBJ BAG61697 BAG38152 BAG11007 BAF83327
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks