BMRB Entry 5456

Title:
1H, 13C, and 15N resonances assignment for the von Willebrand factor A3 domain
Deposition date:
2002-07-08
Original release date:
2002-12-23
Authors:
Nishida, Noritaka; Miyazawa, Mayumi; Sumikawa, Hiromi; Sakakura, Masayoshi; Shimba, Nobuhisa; Takahashi, Hideo; Terasawa, Hiroaki; Suzuki, Ei-ichiro; Shimada, Ichio
Citation:

Citation: Nishida, Noritaka; Miyazawa, Mayumi; Sumikawa, Hiromi; Sakakura, Masayoshi; Shimba, Nobuhisa; Takahashi, Hideo; Terasawa, Hiroaki; Suzuki, Ei-ichiro; Shimada, Ichio. "Letter to the Editor: Backbone 1H, 13C, and 15N resonance assignments of the von Willebrand factor A3 domain"  J. Biomol. NMR 24, 357-358 (2002).

Assembly members:

Assembly members:
von Willebrand factor-A3 domain, polymer, 197 residues, 20861 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts366
15N chemical shifts183
1H chemical shifts183

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1A3 domain1

Entities:

Entity 1, A3 domain 197 residues - 20861 Da.

1   GLYSERMETASPILEALAPROASPCYSSER
2   GLNPROLEUASPVALILELEULEULEUASP
3   GLYSERSERSERPHEPROALASERTYRPHE
4   ASPGLUMETLYSSERPHEALALYSALAPHE
5   ILESERLYSALAASNILEGLYPROARGLEU
6   THRGLNVALSERVALLEUGLNTYRGLYSER
7   ILETHRTHRILEASPVALPROTRPASNVAL
8   VALPROGLULYSALAHISLEULEUSERLEU
9   VALASPVALMETGLNARGGLUGLYGLYPRO
10   SERGLNILEGLYASPALALEUGLYPHEALA
11   VALARGTYRLEUTHRSERGLUMETHISGLY
12   ALAARGPROGLYALASERLYSALAVALVAL
13   ILELEUVALTHRASPVALSERVALASPSER
14   VALASPALAALAALAASPALAALAARGSER
15   ASNARGVALTHRVALPHEPROILEGLYILE
16   GLYASPARGTYRASPALAALAGLNLEUARG
17   ILELEUALAGLYPROALAGLYASPSERASN
18   VALVALLYSLEUGLNARGILEGLUASPLEU
19   PROTHRMETVALTHRLEUGLYASNSERPHE
20   LEUHISLYSLEUCYSSERGLY

Samples:

sample_1: von Willebrand factor-A3 domain, [U-98% 13C; U-98% 15N], 0.5 – 0.8 mM

sample_2: von Willebrand factor-A3 domain, [U-98% 2H; U-98% 13C; U-98% 15N], 0.8 mM

Ex_cond_1: pH: 6.0; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D HN(CO)CAnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAG59985

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks