BMRB Entry 5397

Title:
1H, 13C and 15N resonance assignment of an affibody-target complex
Deposition date:
2002-06-14
Original release date:
2002-12-23
Authors:
Lendel, Christofer; Wahlberg, Elisabet; Berglund, Helena; Eklund, Malin; Nygren, Per-Ake; Hard, Torleif
Citation:

Citation: Lendel, Christofer; Wahlberg, Elisabet; Berglund, Helena; Eklund, Malin; Nygren, Per-Ake; Hard, Torleif. "Letter to the Editor: 1H, 13C and 15N resonance assignments of an affibody-target complex "  J. Biomol. NMR 24, 271-272 (2002).

Assembly members:

Assembly members:
Z-domain, polymer, 58 residues, Formula weight is not available
ZSPA-1, polymer, 58 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts852
13C chemical shifts500
15N chemical shifts134

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Z-domain1
2ZSPA-1 affibody2

Entities:

Entity 1, Z-domain 58 residues - Formula weight is not available

1   VALASPASNLYSPHEASNLYSGLUGLNGLN
2   ASNALAPHETYRGLUILELEUHISLEUPRO
3   ASNLEUASNGLUGLUGLNARGASNALAPHE
4   ILEGLNSERLEULYSASPASPPROSERGLN
5   SERALAASNLEULEUALAGLUALALYSLYS
6   LEUASNASPALAGLNALAPROLYS

Entity 2, ZSPA-1 affibody 58 residues - Formula weight is not available

1   VALASPASNLYSPHEASNLYSGLULEUSER
2   VALALAGLYARGGLUILEVALTHRLEUPRO
3   ASNLEUASNASPPROGLNLYSLYSALAPHE
4   ILEPHESERLEUTRPASPASPPROSERGLN
5   SERALAASNLEULEUALAGLUALALYSLYS
6   LEUASNASPALAGLNALAPROLYS

Samples:

Sample_1: Z-domain, [U-13C; U-15N], 1.5 – 2.0 mM; ZSPA-11.65 – 2.5 mM; potassium phosphate 20 mM; sodium azide 0.01%; D2O10 – 100%

Sample_2: ZSPA-1, [U-13C; U-15N], 1.5 – 2.0 mM; Z-domain1.65 – 2.5 mM; potassium phosphate 20 mM; sodium azide 0.01%; D2O10 – 100%

Conditions: pH*: 5.6; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
CBCANHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available
15N-TOCSY-HSQCnot availablenot availablenot available
15N-NOESY-HSQCnot availablenot availablenot available

Software:

XWIN-NMR - data processing

NMRpipe - data processing

Ansig for Windows - analysis and assignment

NMR spectrometers:

  • Bruker AVANCE 500 MHz
  • Bruker AVANCE 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks