BMRB Entry 5382

Title:
1H, 13C, and 15N backbone resonance assignment of the C-terminal domain of EPSP synthase
Deposition date:
2002-06-04
Original release date:
2002-12-23
Authors:
Kim, Hak; Young, John; Helms, Gregory; Evans, Jeremy
Citation:

Citation: Kim, Hak; Young, John; Helms, Gregory; Evans, Jeremy. "Letter to the Editor: 1H, 13C, and 15N backbone resonance assignments of the C-terminal domain of 5'-enolpyruvylshikimate-3-phosphate synthase"  J. Biomol. NMR 24, 269-270 (2002).

Assembly members:

Assembly members:
EPSP synthase, polymer, 211 residues, 22980 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts384
15N chemical shifts194
1H chemical shifts404

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1C-terminal domain of EPSP synthase1

Entities:

Entity 1, C-terminal domain of EPSP synthase 211 residues - 22980 Da.

1   METGLUSERLEUTHRLEUGLNPROILEALA
2   ARGVALASPGLYTHRILEASNLEUPROGLY
3   SERGLYGLYGLUGLYASPALASERSERALA
4   SERTHRPHELEUALAALAALAALAILELYS
5   GLYGLYTHRVALLYSVALTHRGLYILEGLY
6   ARGASNSERMETGLNGLYASPILEARGPHE
7   ALAASPVALLEUGLULYSMETGLYALATHR
8   ILECYSTRPGLYASPASPTHRILESERCYS
9   THRARGGLYGLULEUASNALAILEASPMET
10   ASPMETASNHISILEPROASPALAALAMET
11   THRILEALATHRALAALALEUPHEALALYS
12   GLYTHRTHRTHRLEUARGASNILETHRASN
13   TRPARGVALLYSGLUTHRASPARGLEUPHE
14   ALAMETALATHRGLULEUARGLYSVALGLY
15   ALAGLUVALGLUGLUGLYHISASPTHRILE
16   ARGILETHRPROPROGLULYSLEUASNPHE
17   ALAGLUILEALATHRTHRASNASPHISARG
18   METALAMETCYSPHESERLEUVALALALEU
19   SERASPTHRPROVALTHRILELEUASPPRO
20   LYSCYSTHRALALYSTHRPHEPROASPTHR
21   PHEGLUGLNLEUALAARGILESERGLNALA
22   ALA

Samples:

sample_1: EPSP synthase, [U-95% 13C; U-90% 15N], 1.0 mM; Tris 10 mM; DTT 1 mM; NaN3 3 mM; D2O 50%

C-term_cond_1: pH: 7.8; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks