BMRB Entry 5374

Title:
Assignment of 1H and 15N resonances and secondary structure of the recombinant RicC3 of 2S albumin storage protein from ricinus communis
Deposition date:
2002-05-13
Original release date:
2003-01-06
Authors:
Pantoja-Uceda, David; Bruix, Marta; Varela, Javier; Lopez-Lucendo, Maria; Gimenez-Gallego, Guillermo; Rico, Manuel; Santoro, Jorge
Citation:

Citation: Pantoja-Uceda, David; Bruix, Marta; Varela, Javier; Lopez-Lucendo, Maria; Gimenez-Gallego, Guillermo; Rico, Manuel; Santoro, Jorge. "Letter to the Editor: Assignment of 1H and 15N resonances and secondary structure of the recombinant RicC3 of 2S albumin storage protein from ricinus communis"  J. Biomol. NMR 23, 331-332 (2002).

Assembly members:

Assembly members:
2S albumin storage protein from Ricinus communis, polymer, 125 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: castor bean   Taxonomy ID: 3988   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Ricinus communis

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts126
1H chemical shifts829
coupling constants99

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RicC3 recombinant1

Entities:

Entity 1, RicC3 recombinant 125 residues - Formula weight is not available

1   ALAGLUPHEMETGLUSERLYSGLYGLUARG
2   GLUGLYSERSERSERGLNGLNCYSARGGLN
3   GLUVALGLNARGLYSASPLEUSERSERCYS
4   GLUARGTYRLEUARGGLNSERSERSERARG
5   ARGSERTHRGLYGLUGLUVALLEUARGMET
6   PROGLYASPGLUASNGLNGLNGLNGLUSER
7   GLNGLNLEUGLNGLNCYSCYSASNGLNVAL
8   LYSGLNVALARGASPGLUCYSGLNCYSGLU
9   ALAILELYSTYRILEALAGLUASPGLNILE
10   GLNGLNGLYGLNLEUHISGLYGLUGLUSER
11   GLUARGVALALAGLNARGALAGLYGLUILE
12   VALSERSERCYSGLYVALARGCYSMETARG
13   GLNTHRARGTHRASN

Samples:

sample1: 2S albumin storage protein from Ricinus communis, [U-15N], 2.0 mM

sample2: 2S albumin storage protein from Ricinus communis 2.0 mM

conditions-1: pH: 3.5; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
1H NOESYnot availablenot availablenot available
1H TOCSYnot availablenot availablenot available
1H COSYnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available
1H-15N NOESY-HSQCnot availablenot availablenot available
1H-15N TOCSY-HSQCnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available

Software:

NMRView v413-504 - data analysis

NMRPipe - processing

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks