BMRB Entry 5256

Title:
Solution Structure of the Unmodified Anticodon Stem-loop from E. coli tRNA(Phe)
Deposition date:
2002-01-11
Original release date:
2002-09-10
Authors:
Cabello-Villegas, J.; Winkler, M.; Nikonowicz, E.
Citation:

Citation: Cabello-Villegas, J.; Winkler, M.; Nikonowicz, E.. "Solution Conformations of Unmodified and A(37)N(6)-dimethylallyl Modified Anticodon Stem-loops of Escherichia coli tRNA (Phe)"  J. Mol. Biol. 319, 1015-1034 (2002).
PubMed: 12079344

Assembly members:

Assembly members:
anticodon stem-loop tRNA(Phe), polymer, 17 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: enzymatic semisynthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
anticodon stem-loop tRNA(Phe): GGGGAUUGAAAAUCCCC

Data sets:
Data typeCount
13C chemical shifts110
15N chemical shifts16
31P chemical shifts17

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1anticodon stem-loop of tRNA(PHE)1

Entities:

Entity 1, anticodon stem-loop of tRNA(PHE) 17 residues - Formula weight is not available

1   GGGGAUUGAA
2   AAUCCCC

Samples:

sample_1: anticodon stem-loop tRNA(Phe), [U-98% 15N], 2 mM; NaCl 10.0 mM; KPi 10.0 mM

sample_2: anticodon stem-loop tRNA(Phe), [U-95% 13C], 1.0 – 2.3 mM; NaCl 10.0 mM; KPi 10.0 mM

sample_cond_1: ionic strength: 20 mM; pH: 6.8; pressure: 1 atm; temperature: 285 K

sample_cond_2: ionic strength: 20 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available
HNN-COSYnot availablenot availablenot available
P31-1H HetCornot availablenot availablenot available

Software:

X-PLOR v3.861 & 3.1 - refinement, structure solution

FELIX v98 - data analysis

NMR spectrometers:

  • Bruker AMX 500 MHz