BMRB Entry 5226

Title:
Letter to the Editor: 1H, 15N and 13C resonance assignments and secondary structure determination of the Ssh10b from Hyperthermphilic Archaeon Sulfolobus shibatae
Deposition date:
2001-12-07
Original release date:
2003-11-25
Authors:
Tong, Yu-Feng; Cui, Qiu; Feng, Yingang; Huang, Li; Wang, Jin-Feng
Citation:

Citation: Tong, Yu-Feng; Cui, Qiu; Feng, Yingang; Huang, Li; Wang, Jin-Feng. "Letter to the Editor: 1H, 15N and 13C resonance assignments and secondary structure of the Ssh10b from Hyperthermphilic Archaeon Sulfolobus shibatae "  J. Biomol. NMR 22, 385-386 (2002).
PubMed: 12018492

Assembly members:

Assembly members:
Ssh10b, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Sulfolobus shibatae   Taxonomy ID: 2286   Superkingdom: Archaea   Kingdom: Crenarchaeota   Genus/species: Sulfolobus shibatae

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts701
13C chemical shifts420
15N chemical shifts101

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ssh10b subunit 11
2Ssh10b subunit 21

Entities:

Entity 1, Ssh10b subunit 1 97 residues - Formula weight is not available

1   METSERSERGLYTHRPROTHRPROSERASN
2   VALVALLEUILEGLYLYSLYSPROVALMET
3   ASNTYRVALLEUALAALALEUTHRLEULEU
4   ASNGLNGLYVALSERGLUILEVALILELYS
5   ALAARGGLYARGALAILESERLYSALAVAL
6   ASPTHRVALGLUILEVALARGASNARGPHE
7   LEUPROASPLYSILEGLUILELYSGLUILE
8   ARGVALGLYSERGLNVALVALTHRSERGLN
9   ASPGLYARGGLNSERARGVALSERTHRILE
10   GLUILEALAILEARGLYSLYS

Samples:

sample_1: Ssh10b, [U-95% 13C; U-98% 15N], 1.5 mM

Ex-cond_1: pH: 5.0 na; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCACOnot availablenot availablenot available
HNCOnot availablenot availablenot available
HBANNHnot availablenot availablenot available
HBA(CO)NHnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
P(i+1)not availablenot availablenot available
P(i-1)not availablenot availablenot available
P(15Ni,1Hi+1)not availablenot availablenot available
P(15Ni,1Hi-1)not availablenot availablenot available

Software:

Felix v98.0 - data processing, peak assignments

NMR spectrometers:

  • Bruker AvanceDMX 600 MHz

Related Database Links:

BMRB 6019
PDB
EMBL CAA67066 CAC12668
GB AAK41236 ACP35357 ACP38017 ACP45513 ACP48689
REF NP_342446 WP_009992406 WP_010923153 YP_002829315 YP_002832002
SP P60848 P60849
AlphaFold P60848 P60849

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks