BMRB Entry 50298

Title:
phosphorylated BAF
Deposition date:
2020-05-26
Original release date:
2020-06-27
Authors:
Marcelot, Agathe; Zinn-Justin, Sophie
Citation:

Citation: Marcelot, Agathe; Petitalot, Ambre; Ropars, Virginie; Le Du, Marie-Helene; Samson, Camille; Dubois, Stevens; Hoffmann, Guillaume; Miron, Simona; Cuniasse, Philippe; Marquez, Jose Antonio; Thai, Robert; Theillet, Francois-Xavier; Zinn-Justin, Sophie. "Di-phosphorylated BAF shows altered structural dynamics and binding to DNA, but interacts with its nuclear envelope partners."  Nucleic Acids Res. 49, 3841-3855 (2021).
PubMed: 33744941

Assembly members:

Assembly members:
entity_1, polymer, 89 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-M13

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts258
15N chemical shifts86
1H chemical shifts273

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1pBAF1

Entities:

Entity 1, pBAF 89 residues - Formula weight is not available

1   GLYTHRTPOSEPGLNLYSHISARGASPPHE
2   VALALAGLUPROMETGLYGLULYSPROVAL
3   GLYSERLEUALAGLYILEGLYGLUVALLEU
4   GLYLYSLYSLEUGLUGLUARGGLYPHEASP
5   LYSALATYRVALVALLEUGLYGLNPHELEU
6   VALLEULYSLYSASPGLUASPLEUPHEARG
7   GLUTRPLEULYSASPTHRALAGLYALAASN
8   ALALYSGLNSERARGASPALAPHEGLYALA
9   LEUARGGLUTRPALAASPALAPHELEU

Samples:

sample_1: pBAF, [U-98% 15N], 350 uM; D2O 10%; DSS 1 uM; potassium phosphate 40 uM; sodium chloride 150 uM

sample_conditions_1: ionic strength: 200 mM; pH: 7.2; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

CcpNmr Analysis - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks