BMRB Entry 50255

Title:
hnRNPA2 1-189 310K
Deposition date:
2020-05-01
Original release date:
2020-08-20
Authors:
Ryan, Veronica; Naik, Mandar; Fawzi, Nicolas
Citation:

Citation: Ryan, Veronica; Watters, Scott; Amaya, Joshua; Khatiwada, Balabhadra; Venditti, Vincenzo; Naik, Mandar; Fawzi, Nicolas. "Weak binding to the A2RE RNA rigidifies hnRNPA2 RRMs and reduces liquid-liquid phase separation and aggregation"  Nucleic Acids Res 48, 10542-10554 (2020).
PubMed: 32870271

Assembly members:

Assembly members:
entity_1, polymer, 189 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJ411

Data sets:
Data typeCount
15N chemical shifts164
1H chemical shifts164

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hnRNPA2 1-1891

Entities:

Entity 1, hnRNPA2 1-189 189 residues - Formula weight is not available

1   METGLUARGGLULYSGLUGLNPHEARGLYS
2   LEUPHEILEGLYGLYLEUSERPHEGLUTHR
3   THRGLUGLUSERLEUARGASNTYRTYRGLU
4   GLNTRPGLYLYSLEUTHRASPCYSVALVAL
5   METARGASPPROALASERLYSARGSERARG
6   GLYPHEGLYPHEVALTHRPHESERSERMET
7   ALAGLUVALASPALAALAMETALAALAARG
8   PROHISSERILEASPGLYARGVALVALGLU
9   PROLYSARGALAVALALAARGGLUGLUSER
10   GLYLYSPROGLYALAHISVALTHRVALLYS
11   LYSLEUPHEVALGLYGLYILELYSGLUASP
12   THRGLUGLUHISHISLEUARGASPTYRPHE
13   GLUGLUTYRGLYLYSILEASPTHRILEGLU
14   ILEILETHRASPARGGLNSERGLYLYSLYS
15   ARGGLYPHEGLYPHEVALTHRPHEASPASP
16   HISASPPROVALASPLYSILEVALLEUGLN
17   LYSTYRHISTHRILEASNGLYHISASNALA
18   GLUVALARGLYSALALEUSERARGGLNGLU
19   METGLNGLUVALGLNSERSERARGSER

Samples:

sample_1: hnRNPA2 1-189, [U-99% 15N], 200 uM; D2O, [U-99% 2H], 10%; NaCl 150 mM; NaPi 20 mM

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks