BMRB Entry 50244

Title:
[r(UGGUGG)(2'OMeU)]4 G-quadruplex
Deposition date:
2020-04-24
Original release date:
2020-05-20
Authors:
Andralojc, Witold
Citation:

Citation: Andralojc, Witold; Pasternak, Karol; Sarzynska, Joanna; Zielinska, Karolina; Kierzek, Ryszard; Gdaniec, Zofia. "The origin of the high stability of 3'-terminal uridine tetrads: contributions of hydrogen bonding, stacking interactions, and steric factors evaluated using modified oligonucleotide analogs"  RNA 26, 2000-2016 (2020).
PubMed: 32967936

Assembly members:

Assembly members:
entity_1, polymer, 7 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: UGGUGGX

Data sets:
Data typeCount
1H chemical shifts50
31P chemical shifts7

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1[r(UGGUGG)(2'OMeU)]4 G-quadruplex1

Entities:

Entity 1, [r(UGGUGG)(2'OMeU)]4 G-quadruplex 7 residues - Formula weight is not available

The X residue is 2'-O-methyl-uridine; the protons belonging to the 2'-O-methyl group are marked as H2'OMe in the uploaded CS file.

1   UGGUGGX

Samples:

sample_1: [r(UGGUGG)(2'OMeU)]4 G-quadruplex 1 mM; sodium phosphate 10 mM; sodium chloride 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-31H COSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker Avance III 700 MHz