BMRB Entry 50216

Title:
HR1c domain from PRK1
Deposition date:
2020-03-25
Original release date:
2020-06-09
Authors:
Sophocleous, Georgios; Mott, Helen
Citation:

Citation: Sophocleous, Georgios; Wood, George; Owen, Darerca; Mott, Helen. "1 H, 15 N and 13 C Resonance Assignments of the HR1c Domain of PRK1, a Protein Kinase C-related Kinase"  Biomol. NMR Assignments 14, 245-250 (2020).
PubMed: 32500230

Assembly members:

Assembly members:
entity_1, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGex6P

Data sets:
Data typeCount
13C chemical shifts346
15N chemical shifts102
1H chemical shifts730

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PRK1 HR1c1

Entities:

Entity 1, PRK1 HR1c 104 residues - Formula weight is not available

1   GLYPROLEUGLYSERHISMETASPTHRGLN
2   GLYSERPROASPLEUGLYALAVALGLULEU
3   ARGILEGLUGLULEUARGHISHISPHEARG
4   VALGLUHISALAVALALAGLUGLYALALYS
5   ASNVALLEUARGLEULEUSERALAALALYS
6   ALAPROASPARGLYSALAVALSERGLUALA
7   GLNGLULYSLEUTHRGLUSERASNGLNLYS
8   LEUGLYLEULEUARGGLUALALEUGLUARG
9   ARGLEUGLYGLULEUPROALAASPHISPRO
10   LYSGLYARGLEULEUARGGLUGLULEUALA
11   ALAALASERSER

Samples:

sample_1: PRK1 HR1c domain, [U-100% 15N], 1.6 mM; sodium phosphate 20 mM; sodium chloride 150 mM

sample_2: PRK1 HR1c domain, [U-100% 13C; U-100% 15N], 1.2 mM; sodium phosphate 20 mM; sodium chloride 150 mM

sample_conditions_1: ionic strength: 0.17 M; pH: 7.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1

Software:

CcpNmr Analysis v2.4 - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker Avance 800 MHz

Related Database Links:

UNP Q16512
AlphaFold Q9UD44

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks