BMRB Entry 50214

Name: 1HN and 15N assignment of PhoQ PD at pH 6.5
Published: 2020-04-21

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50214

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1HN and 15N assignment of PhoQ PD at pH 6.5

Deposition date:

2020-03-18

Original release date:

2020-04-21

Authors:

Hicks, Kevin; Scott, Delbecq; Enea, Sancho-Vaello; Marie-Pierre, Blanc; Katja, Dove; Lynne, Prost; Margaret, Daley; Kornelius, Zeth; Rachel, Klevit; Samuel, Miller

Citation:

Citation: Hicks, Kevin; Delbecq, Scott; Sancho-Vaello, Enea; Blanc, Marie-Pierre; Dove, Katja; Prost, Lynne; Daley, Margaret; Zeth, Kornelius; Klevit, Rachel; Miller, Samuel. "Acidic pH and divalent cation sensing by PhoQ are dispensable for systemic salmonellae virulence." Elife 4, e06792-e06792 (2015).
PubMed: 26002083

Assembly members:

Assembly members:
entity_1, polymer, 153 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Salmonella typhimurium Taxonomy ID: 28901 Superkingdom: Bacteria Kingdom: not available Genus/species: Salmonella enterica

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a-phoQ 45-190-(His)6

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MDKTTFRLLRGESNLFYTLA KWENNKISVELPENLDMQSP TMTLIYDETGKLLWTQRNIP WLIKSIQPEWLKTNGFHEIE TNVDATSTLLSEDHSAQEKL KEVREDDDDAEMTHSVAVNI YPATARMPQLTIVVVDTIPI ELKRSYMHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	125
1H chemical shifts	125

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PhoQ_PD	1

Entities:

Entity 1, PhoQ_PD 153 residues - Formula weight is not available

1

MET

ASP

LYS

THR

PHE

ARG

LEU

ARG

2

GLY

GLU

SER

ASN

LEU

PHE

TYR

THR

LEU

ALA

3

LYS

TRP

GLU

ASN

LYS

ILE

SER

VAL

GLU

4

LEU

PRO

GLU

ASN

LEU

ASP

MET

GLN

SER

PRO

5

THR

MET

THR

LEU

ILE

TYR

ASP

GLU

THR

GLY

6

LYS

LEU

TRP

THR

GLN

ARG

ASN

ILE

PRO

7

TRP

LEU

ILE

LYS

SER

ILE

GLN

PRO

GLU

TRP

8

LEU

LYS

THR

ASN

GLY

PHE

HIS

GLU

ILE

GLU

9

THR

ASN

VAL

ASP

ALA

THR

SER

THR

LEU

10

SER

GLU

ASP

HIS

SER

ALA

GLN

GLU

LYS

LEU

11

LYS

GLU

VAL

ARG

GLU

ASP

ALA

12

GLU

MET

THR

HIS

SER

VAL

ALA

VAL

ASN

ILE

13

TYR

PRO

ALA

THR

ALA

ARG

MET

PRO

GLN

LEU

14

THR

ILE

VAL

ASP

THR

ILE

PRO

ILE

15

GLU

LEU

LYS

ARG

SER

TYR

MET

HIS

16

HIS

Samples:

sample_1: PhoQ_PD, [U-100% 15N], 400 uM; sodium phosphate 20 mM; sodium chloride 150 mM; magnesium chloride 20 mM; EDTA 20 mM

sample_conditions_1: ionic strength: 0.2 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr Analysis - data analysis

NMR spectrometers:

Bruker Avance 700 MHz

UNP	D0ZV89.1
AlphaFold	D0ZV89