BMRB Entry 50208

Title:
Purification of native CCL7 and its functional interaction with selected chemokine receptors
Deposition date:
2020-03-07
Original release date:
2020-08-25
Authors:
Goncharuk, Marina; Roy, Debarati; Dubinnyi, Maxim; Nadezhdin, Kirill; Srivastava, Ashish; Baidya, Mithu; Dwivedi-Agnihotri, Hemlata; Arseniev, Alexander; Shukla, Arun
Citation:

Citation: Goncharuk, Marina; Roy, Debarati; Dubinnyi, Maxim; Nadezhdin, Kirill; Srivastava, Ashish; Baidya, Mithu; Dwivedi-Agnihotri, Hemlata; Arseniev, Alexander; Shukla, Arun. "Purification of native CCL7 and its functional interaction with selected chemokine receptors"  Protein Expr. Purif. 171, 105617-105617 (2020).
PubMed: 32145391

Assembly members:

Assembly members:
entity_1, polymer, 85 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEMEX-1(his6)

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts84
1H chemical shifts536

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HisCCL71

Entities:

Entity 1, HisCCL7 85 residues - Formula weight is not available

1   METHISHISHISHISHISHISGLYSERGLN
2   PROVALGLYILEASNTHRSERTHRTHRCYS
3   CYSTYRARGPHEILEASNLYSLYSILEPRO
4   LYSGLNARGLEUGLUSERTYRARGARGTHR
5   THRSERSERHISCYSPROARGGLUALAVAL
6   ILEPHELYSTHRLYSLEUASPLYSGLUILE
7   CYSALAASPPROTHRGLNLYSTRPVALGLN
8   ASPPHEMETLYSHISLEUASPLYSLYSTHR
9   GLNTHRPROLYSLEU

Samples:

sample_1: His6-tagged human chemokine CCL7, [U-100% 15N], 0.8 mM; KPi 20 mM; MES 0.4 mM; NaCl 7 mM

sample_conditions_1: ionic strength: 0.007 M; pH: 5.1; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.0 - collection

CARA v1.9.1.7 - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance III 800 MHz

Related Database Links:

SP P80098
AlphaFold Q569J6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks