BMRB Entry 50199

Name: 1H, 15N Resonance Assignments of VAMP2(1-96) in HEK-293T Cells
Published: 2020-02-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50199

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N Resonance Assignments of VAMP2(1-96) in HEK-293T Cells

Deposition date:

2020-02-18

Original release date:

2020-02-19

Authors:

Wang, Chuchu; Tu, Jia; Zhang, Shengnan; Li, Dan; Liu, Cong

Citation:

Citation: Wang, Chuchu; Tu, Jia; Zhang, Shengnan; Cai, Bin; Liu, Zhenying; Hou, Shouqiao; Zhong, Qinglu; Hu, Xiao; Liu, Wenbin; Li, Guohui; Liu, Zhijun; He, Lin; Diao, Jiajie; Zhu, Zheng-Jiang; Li, Dan; Liu, Cong. "Different regions of synaptic vesicle membrane regulate VAMP2 conformation for the SNARE assembly" Nat. Commun. 11, 1531-1531 (2020).
PubMed: 32210233

Assembly members:

Assembly members:
entity_1, polymer, 96 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pDC191

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSATAATVPPAAPAGEGGPP APPPNLTSNRRLQQTQAQVD EVVDIMRVNVDKVLERDQKL SELDDRADALQAGASQFETS AAKLKRKYWWKNLKMM

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	69
1H chemical shifts	69

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	VAMP2(1-96) in HEK-293T Cells	1

Entities:

Entity 1, VAMP2(1-96) in HEK-293T Cells 96 residues - Formula weight is not available

1

MET

SER

ALA

THR

ALA

THR

VAL

PRO

2

ALA

PRO

ALA

GLY

GLU

GLY

PRO

3

ALA

PRO

ASN

LEU

THR

SER

ASN

ARG

4

ARG

LEU

GLN

THR

GLN

ALA

GLN

VAL

ASP

5

GLU

VAL

ASP

ILE

MET

ARG

VAL

ASN

VAL

6

ASP

LYS

VAL

LEU

GLU

ARG

ASP

GLN

LYS

LEU

7

SER

GLU

LEU

ASP

ARG

ALA

ASP

ALA

LEU

8

GLN

ALA

GLY

ALA

SER

GLN

PHE

GLU

THR

SER

9

ALA

LYS

LEU

LYS

ARG

LYS

TYR

TRP

10

LYS

ASN

LEU

LYS

MET

Samples:

sample_1: VAMP2(1-96) in HEK-293T Cells, [U-100% 15N], 0.08 ± 0.01 mM

sample_conditions_1: ionic strength: 0.158 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe v8.9 - processing

SPARKY v3.115 - chemical shift assignment, chemical shift calculation

NMR spectrometers:

Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks