BMRB Entry 50187

Title:
RBM5 RRM1-Zf1
Deposition date:
2020-02-02
Original release date:
2020-06-09
Authors:
Soni, Komal; Sattler, Michael
Citation:

Citation: Soni, Komal; Martinez-Lumbreras, Santiago; Sattler, Michael. "Conformational Dynamics From Ambiguous Zinc Coordination in the RanBP2-Type Zinc Finger of RBM5"  J. Mol. Biol. 432, 4127-4138 (2020).
PubMed: 32450081

Assembly members:

Assembly members:
entity_1, polymer, . residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts321
15N chemical shifts110
1H chemical shifts109

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RRM1-Zf11
2Zn2

Entities:

Entity 1, RRM1-Zf1 - Formula weight is not available

Sequence numbering starts from 92 and residues 92, 93 are cloning artifacts.

1   METGLYGLUARGGLUSERLYSTHRILEMET
2   LEUARGGLYLEUPROTHRTHRILETHRGLU
3   SERASPILEARGGLUMETMETGLUSERPHE
4   GLUGLYPROGLNPROALAASPVALARGLEU
5   METLYSARGLYSTHRGLYVALSERARGGLY
6   PHEALAPHEVALGLUPHETYRHISLEUGLN
7   ASPALATHRSERTRPMETGLUALAASNGLN
8   LYSLYSLEUVALILEGLNGLYLYSHISILE
9   ALAMETHISTYRSERASNPROARGPROLYS
10   PHEGLUASPTRPLEUCYSASNLYSCYSCYS
11   LEUASNASNPHEARGLYSARGLEULYSCYS
12   PHEARGCYSGLYALAASPLYSPHEASP

Entity 2, Zn - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: entity_1, [U-13C; U-15], mM; MES 20 mM; sodium chloride 400 mM; DTT 1 mM

sample_conditions_1: ionic strength: 0.4 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

Analysis, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

NCBI NP_005769.1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks