BMRB Entry 50153

Name: Isolation and characterization of antimicrobial peptides with unusual disulfide connectivity from the colonial ascidian Synoicum turgens [Turgencin B(Mox2)]
Published: 2020-06-09

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50153

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Isolation and characterization of antimicrobial peptides with unusual disulfide connectivity from the colonial ascidian Synoicum turgens [Turgencin B(Mox2)]

Deposition date:

2020-01-09

Original release date:

2020-06-09

Authors:

Hansen, Ida; Isaksson, Johan; Poth, Aaron; Andersen, Aaron; Hansen, Kine; Richard, Celine; Blencke, Hans-Matti; Stensvaag, Klara; Craik, David; Tor, Haug

Citation:

Citation: Hansen, Ida; Isaksson, Johan; Poth, Aaron; Hansen, Kine; Andersen, Aaron; Richard, Celine; Blencke, Hans-Matti; Stensvag, Klara; Craik, David; Haug, Tor. "Isolation and characterization of antimicrobial peptides with unusual disulfide connectivity from the colonial ascidian Synoicum turgens" Mar. Drugs 18, .-. (2020).
PubMed: 31940927

Assembly members:

Assembly members:
Turgencin B (Mox2), polymer, 36 residues, 3575 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: 562744 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Synoicum turgens

Experimental source:

Experimental source: Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Turgencin B (Mox2): GIKEMLCNMACAQTVCKKSG GPLCDTCQAACKALGX

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1_1

Data type	Count
13C chemical shifts	104
15N chemical shifts	35
1H chemical shifts	223

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Turgencin B (Mox2)	1

Entities:

Entity 1, Turgencin B (Mox2) 36 residues - 3575 Da.

Methionines are predominantly oxidized

1

GLY

ILE

LYS

GLU

MET

LEU

CYS

ASN

MET

ALA

2

CYS

ALA

GLN

THR

VAL

CYS

LYS

SER

GLY

3

GLY

PRO

LEU

CYS

ASP

THR

CYS

GLN

ALA

4

CYS

LYS

ALA

LEU

GLY

NH2

Samples:

sample_1_1: entity_1 2 mg

sample_conditions_1_1: pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1_1	isotropic	sample_conditions_1_1
2D 1H-15N HSQC	sample_1_1	isotropic	sample_conditions_1_1
2D 1H-1H TOCSY	sample_1_1	isotropic	sample_conditions_1_1
2D 1H-13C HSQC	sample_1_1	isotropic	sample_conditions_1_1
2D 1H-1H COSY	sample_1_1	isotropic	sample_conditions_1_1
2D 1H-13C HMBC	sample_1_1	isotropic	sample_conditions_1_1
2D 1H-13C TOCSYHSQC	sample_1_1	isotropic	sample_conditions_1_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks