BMRB Entry 50138

Title:
NMR insights into the pre-amyloid ensemble and secretion targeting of the curli subunit CsgA
Deposition date:
2019-12-18
Original release date:
2020-06-09
Authors:
Sewell, Lee; Stylianou, Fisentzos; Xu, Yingqi; Taylor, Jon; Sefer, Lea; Matthews, Steve
Citation:

Citation: Sewell, Lee; Stylianou, Fisentzos; Xu, Yingqi; Taylor, Jon; Sefer, Lea; Matthews, Steve. "NMR Insights Into the Pre-Amyloid Ensemble and Secretion Targeting of the Curli Subunit CsgA"  Sci. Rep. 10, 7896-7896 (2020).
PubMed: 32398666

Assembly members:

Assembly members:
entity_1, polymer, 134 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28a

Data sets:
Data typeCount
13C chemical shifts301
15N chemical shifts117
1H chemical shifts117

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pre-fibrillar CsgA monomer1

Entities:

Entity 1, Pre-fibrillar CsgA monomer 134 residues - Formula weight is not available

1   METGLYVALVALPROGLNTYRGLYGLYGLY
2   GLYASNHISGLYGLYGLYGLYASNASNSER
3   GLYPROASNSERGLULEUASNILETYRGLN
4   TYRGLYGLYGLYASNSERALALEUALALEU
5   GLNTHRASPALAARGASNSERASPLEUTHR
6   ILETHRGLNHISGLYGLYGLYASNGLYALA
7   ASPVALGLYGLNGLYSERASPASPSERSER
8   ILEASPLEUTHRGLNARGGLYPHEGLYASN
9   SERALATHRLEUASPGLNTRPASNGLYLYS
10   ASNSERGLUMETTHRVALLYSGLNPHEGLY
11   GLYGLYASNGLYALAALAVALASPGLNTHR
12   ALASERASNSERSERVALASNVALTHRGLN
13   VALGLYPHEGLYASNASNALATHRALAHIS
14   GLNTYRLEUGLN

Samples:

sample_1: entity_1, [U-13C; U-15N], mM; sodium phosphate 20 mM

sample_conditions_1: pH: 7.2; pressure: 1 atm; temperature: 283.2 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 950 MHz
  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks