BMRB Entry 50121

Title:
1H, 15N assignments of single-stranded DNA binding domains from the 70 kDa subunit of Human Replication Protein A bound to ssDNA
Deposition date:
2019-12-10
Original release date:
2019-12-20
Authors:
Arunkumar, Alphonse
Citation:

Citation: Arunkumar, Alphonse; Stauffer, Melissa; Bochkareva, Elena; Bochkarev, Alexey; Chazin, Walter. "Independent and Coordinated Functions of Replication Protein A Tandem High Affinity Single-stranded DNA Binding Domains"  J. Biol. Chem. 278, 41077-41082 (2003).
PubMed: 12881520

Assembly members:

Assembly members:
entity_1, polymer, 242 residues, 27071 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSV281

Data sets:
Data typeCount
15N chemical shifts191
1H chemical shifts191

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RPA70AB1

Entities:

Entity 1, RPA70AB 242 residues - 27071 Da.

1   GLNSERLYSVALVALPROILEALASERLEU
2   THRPROTYRGLNSERLYSTRPTHRILECYS
3   ALAARGVALTHRASNLYSSERGLNILEARG
4   THRTRPSERASNSERARGGLYGLUGLYLYS
5   LEUPHESERLEUGLULEUVALASPGLUSER
6   GLYGLUILEARGALATHRALAPHEASNGLU
7   GLNVALASPLYSPHEPHEPROLEUILEGLU
8   VALASNLYSVALTYRTYRPHESERLYSGLY
9   THRLEULYSILEALAASNLYSGLNPHETHR
10   ALAVALLYSASNASPTYRGLUMETTHRPHE
11   ASNASNGLUTHRSERVALMETPROCYSGLU
12   ASPASPHISHISLEUPROTHRVALGLNPHE
13   ASPPHETHRGLYILEASPASPLEUGLUASN
14   LYSSERLYSASPSERLEUVALASPILEILE
15   GLYILECYSLYSSERTYRGLUASPALATHR
16   LYSILETHRVALARGSERASNASNALAGLU
17   VALALALYSARGASNILETYRLEUMETASP
18   THRSERGLYLYSVALVALTHRALATHRLEU
19   TRPGLYGLUASPALAASPLYSPHEASPGLY
20   SERARGGLNPROVALLEUALAILELYSGLY
21   ALAARGVALSERASPPHEGLYGLYARGSER
22   LEUSERVALLEUSERSERSERTHRILEILE
23   ALAASNPROASPILEPROGLUALATYRLYS
24   LEUARGGLYTYRPHEASPALAGLUGLYGLY
25   ALALEU

Samples:

sample_1: entity_1, [U-15N], 50 uM; TRIS 20 ± 1 mM; potassium chloride 150 ± 2 mM; glycerol 10 ± 1 % w/v; DTT 1 ± 0.1 mM

sample_conditions_1: ionic strength: 170 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks