BMRB Entry 50095

Title:
1H, 15N chemical shift assignments of the imino groups of yeast tRNAPhe: influence of the post-transcriptional modifications
Deposition date:
2019-11-28
Original release date:
2020-09-19
Authors:
Catala, Marjorie; Gato, Alexandre; Tisne, Carine; Barraud, Pierre
Citation:

Citation: Catala, Marjorie; Gato, Alexandre; Tisne, Carine; Barraud, Pierre. "1H, 15N chemical shift assignments of the imino groups of yeast tRNA Phe: influence of the post-transcriptional modifications"  Biomol. NMR Assignments 14, 169-174 (2020).
PubMed: 32239363

Assembly members:

Assembly members:
unmodified yeast tRNAPhe, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: enzymatic semisynthesis

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts27
1H chemical shifts27

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1tRNAPhe1

Entities:

Entity 1, tRNAPhe 76 residues - Formula weight is not available

1   GCGGAUUUAG
2   CUCAGUUGGG
3   AGAGCGCCAG
4   ACUGAAGAUC
5   UGGAGGUCCU
6   GUGUUCGAUC
7   CACAGAAUUC
8   GCACCA

Samples:

sample_1: entity_1 1.8-2.0 mM; sodium phosphate 10 mM; magnesium chloride 10 mM

sample_2: entity_1, [U-15N]-Gua, [U-15N]-Ura, 1.5-1.8 mM; sodium phosphate 10 mM; magnesium chloride 10 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 311 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N BEST-TROSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

TOPSPIN v3.5, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz

Related Database Links:

tRNADB tdbR00000083