BMRB Entry 50084

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR50084

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Title:
E6AP C-lobe
Deposition date:
2019-11-07
Original release date:
2020-07-17
Authors:
Beasley, Steven; Spratt, Donald
Citation:

Citation: Beasley, Steven; Kellum, Chloe; Orlomoski, Rachel; Idrizi, Feston; Spratt, Donald. "An Angelman Syndrome Substitution in the HECT E3 Ubiquitin Ligase C-terminal Lobe of E6AP Affects Protein Stability and Activity"  PLoS ONE 15, e0235925-e0235925 (2020).
PubMed: 32639967

Assembly members:

Assembly members:
entity_1, polymer, 117 residues, 13235.10 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pE6AP-Clobe

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSGSRNLDFQALEETTEYDG GYTRDSVLIREFWEIVHSFT DEQKRLFLQFTTGTDRAPVG GLGKLKMIIAKNGPDTERLP TSHTCFNVLLLPEYSSKEKL KERLLKAITYAKGFGML

Data sets:
Data typeCount
13C chemical shifts524
15N chemical shifts118
1H chemical shifts822

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1E6AP Clobe1

Entities:

Entity 1, E6AP Clobe 117 residues - 13235.10 Da.

1   GLYSERGLYSERARGASNLEUASPPHEGLN
2   ALALEUGLUGLUTHRTHRGLUTYRASPGLY
3   GLYTYRTHRARGASPSERVALLEUILEARG
4   GLUPHETRPGLUILEVALHISSERPHETHR
5   ASPGLUGLNLYSARGLEUPHELEUGLNPHE
6   THRTHRGLYTHRASPARGALAPROVALGLY
7   GLYLEUGLYLYSLEULYSMETILEILEALA
8   LYSASNGLYPROASPTHRGLUARGLEUPRO
9   THRSERHISTHRCYSPHEASNVALLEULEU
10   LEUPROGLUTYRSERSERLYSGLULYSLEU
11   LYSGLUARGLEULEULYSALAILETHRTYR
12   ALALYSGLYPHEGLYMETLEU

Samples:

sample_1: E6AP-Clobe (G761-L875), [U-100% 13C; U-100% 15N], 3.5 mM; MES-NaOH 20 mM; NaCl 120 mM; EDTA 1 mM

sample_conditions_1: ionic strength: 141 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D HCCH-TOCSY aliphaticsample_1isotropicsample_conditions_1
3D HCCH-TOCSY aromaticsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

NMRView vv9.1.0-b63, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking, processing, refinement

NMR spectrometers:

  • Varian INOVA 600 MHz MHz

Related Database Links:

UNIPROT Q05086
AlphaFold Q9UEP9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks