BMRB Entry 50019

Name: CproIAPP in DPC at pH 4.5
Published: 2020-03-03

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50019

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

CproIAPP in DPC at pH 4.5

Deposition date:

2019-09-17

Original release date:

2020-03-03

Authors:

DeLisle, Charles; Alexander, Malooley; Banerjee, Indrani; Lorieau, Justin

Citation:

Citation: DeLisle, Charles; Malooley, Alexander; Banerjee, Indrani; Lorieau, Justin. "Pro-Islet Amyloid Polypeptide in Micelles Contains a Helical Prohormone Segment" FEBS J. 287, 4440-4457 (2020).
PubMed: 32077246

Assembly members:

Assembly members:
CproIAPP, polymer, 16 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CproIAPP: NAVEVLKREPLNYLPL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
1H chemical shifts	130

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CproIAPP	1

Entities:

Entity 1, CproIAPP 16 residues - Formula weight is not available

1

ASN

ALA

VAL

GLU

VAL

LEU

LYS

ARG

GLU

PRO

2

LEU

ASN

TYR

LEU

PRO

LEU

Samples:

sample_1: Cpro 1.0 ± 0.2 mM; DPC, [U-100% 2H], 200 ± 20 mM; sodium acetate 30 ± 3 mM; sodium chloride 50 ± 5 mM; sodium azide 0.03 ± 0.01 % w/w

sample_conditions_1: ionic strength: 50 mM; pH: 4.5; pressure: 1 atm; temperature: 305 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.2, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker AvanceIII 750 MHz