BMRB Entry 50014

Title:
ctSpp2(208-254) backbone assignment
Deposition date:
2019-09-12
Original release date:
2020-09-21
Authors:
Favretto, Filippo; Zweckstetter, Markus
Citation:

Citation: Hamann, Florian; Schmitt, Andreas; Favretto, Filippo; Hofele, Romina; Neumann, Piotr; Xiang, ShengQi; Urlaub, Henning; Zweckstetter, Markus; Ficner, Ralf. "Structural analysis of the intrinsically disordered splicing factor Spp2 and its binding to the DEAH-box ATPase Prp2"  Proc. Natl. Acad. Sci. U.S.A. 117, 2948-2956 (2020).
PubMed: 31974312

Assembly members:

Assembly members:
Molecule 1, polymer, 47 residues, 5226.9257 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 209285   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Chaetomium thermophilum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pASG-IBA 25

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Molecule 1: GPMVDDFGENLLRSFGWDGK MRGKVKEVKRYANLAGLGAR NVKEAED

Data typeCount
13C chemical shifts130
15N chemical shifts45
1H chemical shifts45

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Molecule 11

Entities:

Entity 1, Molecule 1 47 residues - 5226.9257 Da.

1   GLYPROMETVALASPASPPHEGLYGLUASN
2   LEULEUARGSERPHEGLYTRPASPGLYLYS
3   METARGGLYLYSVALLYSGLUVALLYSARG
4   TYRALAASNLEUALAGLYLEUGLYALAARG
5   ASNVALLYSGLUALAGLUASP

Samples:

sample_1: ctSpp2, [15N 13C], 0.52 mM; DTT 1 mM; EDTA 5 mM; HEPES 10 mM; sodium chloride 200 mM; glycerol 0.5 % w/v; sodium azide 0.02 % w/v

sample_conditions_1: ionic strength: 0.2 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

CcpNmr Analysis v2.2, CCPN - chemical shift assignments

DANGLE v1.1, University of Cambridge - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks