BMRB Entry 50008

Name: ngMinE/I24N
Published: 2020-09-21

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50008

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

ngMinE/I24N

Deposition date:

2019-09-03

Original release date:

2020-09-21

Authors:

Cai, Mengli

Citation:

Citation: Cai, Mengli; Huang, Ying; Shen, Yang; Li, Min; Mizuuchi, Michiyo; Ghirlando, Rodolfo; Mizuuchi, Kiyoshi; Clore, G Marius. "Probing transient excited states of the bacterial cell division regulator MinE by relaxation dispersion NMR spectroscopy" Proc. Natl. Acad. Sci. U.S.A. 116, 25446-25455 (2019).
PubMed: 31772021

Assembly members:

Assembly members:
entity_1, polymer, 89 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Neisseria gonorrheae Taxonomy ID: 485 Superkingdom: Bacteria Kingdom: not available Genus/species: Neisseria gonorrheae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Neisseria gonorrheae pet Vector: pet11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSLIELLFGQMQKTATVARD RLQNIIAQERAQEGQTPDYL PTLRKALMEVLSKYVNVSLD NIRISQEKQDGMDVLELNIT LPEQKKVLE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	240
15N chemical shifts	79
1H chemical shifts	79

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ngMinE/I24N	1

Entities:

Entity 1, ngMinE/I24N 89 residues - Formula weight is not available

1

MET

SER

LEU

ILE

GLU

LEU

PHE

GLY

GLN

2

MET

GLN

LYS

THR

ALA

THR

VAL

ALA

ARG

ASP

3

ARG

LEU

GLN

ASN

ILE

ALA

GLN

GLU

ARG

4

ALA

GLN

GLU

GLY

GLN

THR

PRO

ASP

TYR

LEU

5

PRO

THR

LEU

ARG

LYS

ALA

LEU

MET

GLU

VAL

6

LEU

SER

LYS

TYR

VAL

ASN

VAL

SER

LEU

ASP

7

ASN

ILE

ARG

ILE

SER

GLN

GLU

LYS

GLN

ASP

8

GLY

MET

ASP

VAL

LEU

GLU

LEU

ASN

ILE

THR

9

LEU

PRO

GLU

GLN

LYS

VAL

LEU

GLU

Samples:

sample_1: ngMinE/I24N, [U-98% 13C; U-98% 15N], 0.3 mM; Kpi 25 mM; EDTA 0.5 mM

sample_conditions_1: ionic strength: 0.025 M; pH: 6.5; pressure: 1 atm; temperature: 298k K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

PIPP, Garrett - peak picking

NMR spectrometers:

Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks