BMRB Entry 4981

Title:
Identification of the phospholipid binding site of human beta2-glycoprotein I domain V by heteronuclear magnetic resonance
Deposition date:
2001-03-27
Original release date:
2001-05-29
Authors:
Hoshino, Masaru; Hagihara, Yoshihisa; Nishii, Ichiro; Yamazaki, Toshio; Kato, Hisao; Goto, Yuji
Citation:

Citation: Hoshino, Masaru; Hagihara, Yoshihisa; Nishii, Ichiro; Yamazaki, Toshio; Kato, Hisao; Goto, Yuji. "Identification of the phospholipid binding site of human beta2-glycoprotein I domain V by heteronuclear magnetic resonance"  J. Mol. Biol. 304, 927-940 (2000).
PubMed: 11124037

Assembly members:

Assembly members:
beta2-glycoprotein I, polymer, 86 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts610
13C chemical shifts389
15N chemical shifts88

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1beta2-GPI domain V1

Entities:

Entity 1, beta2-GPI domain V 86 residues - Formula weight is not available

1   THRLYSALASERCYSLYSLEUPROVALLYS
2   LYSALATHRVALVALTYRGLNGLYGLUARG
3   VALLYSILEGLNGLULYSPHELYSASNGLY
4   METLEUHISGLYASPLYSVALSERPHEPHE
5   CYSLYSASNLYSGLULYSLYSCYSSERTYR
6   THRGLUASPALAGLNCYSILEASPGLYTHR
7   ILEGLUVALPROLYSCYSPHELYSGLUHIS
8   SERSERLEUALAPHETRPLYSTHRASPALA
9   SERASPVALLYSPROCYS

Samples:

sample_1: beta2-glycoprotein I, [U-15N], 1.0 mM

sample_2: beta2-glycoprotein I, [U-15N; U-13C], 1.0 mM

condition_1: pH: 6.0; temperature: 298 K; ionic strength: 0.12 M

Experiments:

NameSampleSample stateSample conditions
HNCACBnot availablenot availablecondition_1
CBCA(CO)NHnot availablenot availablecondition_1
HN(CO)CAHAnot availablenot availablecondition_1
1H-13C-1H HCCH-TOCSYnot availablenot availablecondition_1
1H-1H-15N NOESYnot availablenot availablecondition_1
13C-1H-1H NOESYnot availablenot availablecondition_1
HNCOnot availablenot availablecondition_1

Software:

nmrPipe v1.7 - processing

pipp v3.9.9 - processing

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 16639
PDB
DBJ BAG36570 BAG60642 BAI46022
EMBL CAA37664 CAA40977 CAA41113 CAA72279
GB AAA51766 AAB21330 AAH20703 AAH26283 AAK71538
REF NP_000033 NP_001009118 XP_001084366 XP_002748690 XP_002827786
SP P02749 Q95LB0
AlphaFold Q95LB0 P02749

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks