BMRB Entry 4898

Title:
Assignment of 1H, 13C, 15N and 31P resonances of the FYVE domain in the complex with phosphatidylinositol 3-phosphate.
Deposition date:
2000-11-13
Original release date:
2005-06-02
Authors:
Kutateladze, Tatiana; Overduin, Michael
Citation:

Citation: Kutateladze, Tatiana; Capelluto, D.; Ferguson, C.; Cheever, M.; Kutateladze, A.; Prestwich, G.; Overduin, Michael. "Multivalent mechanism of membrane insertion by the FYVE domain"  J. Biol. Chem. 279, 3050-3057 (2004).
PubMed: 14578346

Assembly members:

Assembly members:
Early Endosome Antigen 1, polymer, 86 residues, Formula weight is not available
1,2 Dibutanoyl-sn-glycero-3-phospho[1D-myo-inositol], non-polymer, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts616
13C chemical shifts383
15N chemical shifts109
31P chemical shifts2

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FYVE1
2PtdIns3P2
3Zn-13
4Zn-23

Entities:

Entity 1, FYVE 86 residues - Formula weight is not available

1   ALAVALGLNGLULEUGLYARGGLUASNGLN
2   SERLEUGLNILELYSHISTHRGLNALALEU
3   ASNARGLYSTRPALAGLUASPASNGLUVAL
4   GLNASNCYSMETALACYSGLYLYSGLYPHE
5   SERVALTHRVALARGARGHISHISCYSARG
6   GLNCYSGLYASNILEPHECYSALAGLUCYS
7   SERALALYSASNALALEUTHRPROSERSER
8   LYSLYSPROVALARGVALCYSASPALACYS
9   PHEASNASPLEUGLNGLY

Entity 2, PtdIns3P 1 residues - C17 H32 O16 P2 - Formula weight is not available

1   PTDINS3P

Entity 3, Zn-1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Early Endosome Antigen 1, [U-15N], 2.0 mM; 1,2 Dibutanoyl-sn-glycero-3-phospho[1D-myo-inositol] 1.0 mM; ZINC (II) ION 2.0 mM

sample_2: Early Endosome Antigen 1, [U-15N; U-13C], 2.0 mM; 1,2 Dibutanoyl-sn-glycero-3-phospho[1D-myo-inositol] 1.0 mM; ZINC (II) ION 2.0 mM

Ex-cond_1: pH: 6.7; temperature: 298 K; ionic strength: 0.2 M

Experiments:

NameSampleSample stateSample conditions
2D DIPSInot availablenot availablenot available
2D NOESYnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
1H-15N HMQCJnot availablenot availablenot available
1H-13C NOESYnot availablenot availablenot available
H(CCO)NH TOCSYnot availablenot availablenot available
C(CO)NH TOCSYnot availablenot availablenot available
1H-31P HARTMANN-HAHNnot availablenot availablenot available
HNHAnot availablenot availablenot available
13C F1-filtered, F2-edited NOESYnot availablenot availablenot available

Software:

NMRPipe - data processing

PIPP - peak picking

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 500 MHz
  • Varian Mercury 400 MHz

Related Database Links:

REF XP_001926390 XP_001510589 XP_001915836 NP_003557 XP_001104577
SWISS-PROT Q15075
GenBank AAI56546 EAW97471 AAA79121
EMBL CAA55632
PDB
BMRB 4579

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks