BMRB Entry 4811

Title:
1H and 15N Chemical Shift Assignments for Apo-Recombinant Bovine alpha-Lactalbumin
Deposition date:
2000-08-17
Original release date:
2000-11-13
Authors:
Wijesinha-Bettoni, Ramani; Dobson, Christopher; Redfield, Christina
Citation:

Citation: Wijesinha-Bettoni, Ramani; Dobson, Christopher; Redfield, Christina. "Comparison of the Structural and Dynamical Properties of Holo and Apo bovine Alpha-lactalbumin by NMR Spectroscopy"  J. Mol. Biol. 307, 885-898 (2001).
PubMed: 11273708

Assembly members:

Assembly members:
apo bovine alpha-lactalbumin, polymer, 124 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: bovine   Taxonomy ID: 9909   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos Primigenius

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet3a

Data sets:
Data typeCount
15N chemical shifts115
1H chemical shifts217

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apo-BLA1

Entities:

Entity 1, apo-BLA 124 residues - Formula weight is not available

1   METGLUGLNLEUTHRLYSCYSGLUVALPHE
2   ARGGLULEULYSASPLEULYSGLYTYRGLY
3   GLYVALSERLEUPROGLUTRPVALCYSTHR
4   THRPHEHISTHRSERGLYTYRASPTHRGLU
5   ALAILEVALGLNASNASNASPSERTHRGLU
6   TYRGLYLEUPHEGLNILEASNASNLYSILE
7   TRPCYSLYSASNASPGLNASPPROHISSER
8   SERASNILECYSASNILESERCYSASPLYS
9   PHELYSASPASPASPLEUTHRASPASPILE
10   VALCYSVALLYSLYSILELEUASPLYSVAL
11   GLYILEASNTYRTRPLEUALAHISLYSALA
12   LEUCYSSERGLULYSLEUASPGLNTRPLEU
13   CYSGLULYSLEU

Samples:

apo-bla_15N_sample: apo bovine alpha-lactalbumin, [U-15N], 2.0 mM; EDTA 15 mM; NaCl 0.5 M

Ca2+_free_conditions: ionic strength: 0.5 M; pH: 7.1; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCapo-bla_15N_samplenot availableCa2+_free_conditions
3D 1H-15N NOESY-HSQCapo-bla_15N_samplenot availableCa2+_free_conditions
2D 1H pre-TOCSY COSYapo-bla_15N_samplenot availableCa2+_free_conditions

Software:

FELIX v2.30 - data processing

NMR spectrometers:

  • Home-built . 750 MHz

Related Database Links:

BMRB 4332

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks