BMRB Entry 4763

Title:
15N relaxation data and model-free parameters for the N-terminal receiver domain of double-mutant (D86N/A89T) unphosphorylated NtrC receiver domain (NtrCr(D86N/A89T))
Deposition date:
2000-06-14
Original release date:
2002-04-11
Authors:
Kern, D.; Volkman, B.; Wemmer, D.
Citation:

Citation: Volkman, Brian; Lipson, Doron; Wemmer, David; Kern, Dorothee. "Two-state allosteric behavior in a single domain signaling protein"  Science 291, 2429-2433 (2001).

Assembly members:

Assembly members:
Nitrogen regulatory protein C N-terminal receiver domain, polymer, 124 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacteria   Taxonomy ID: 602   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Salmonella typhimurium

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
T1 relaxation values97
T2 relaxation values194

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NITROGEN REGULATION PROTEIN1

Entities:

Entity 1, NITROGEN REGULATION PROTEIN 124 residues - Formula weight is not available

1   METGLNARGGLYILEVALTRPVALVALASP
2   ASPASPSERSERILEARGTRPVALLEUGLU
3   ARGALALEUALAGLYALAGLYLEUTHRCYS
4   THRTHRPHEGLUASNGLYASNGLUVALLEU
5   ALAALALEUALASERLYSTHRPROASPVAL
6   LEULEUSERASPILEARGMETPROGLYMET
7   ASPGLYLEUALALEULEULYSGLNILELYS
8   GLNARGHISPROMETLEUPROVALILEILE
9   METTHRALAHISSERASNLEUASPTHRALA
10   VALSERALATYRGLNGLNGLYALAPHEASP
11   TYRLEUPROLYSPROPHEASPILEASPGLU
12   ALAVALALALEUVALGLUARGALAILESER
13   HISTYRGLNGLU

Samples:

sample_1: Nitrogen regulatory protein C N-terminal receiver domain, [U-99% 15N], 0.9 mM; phosphate buffer 50 mM

sample_cond_1: ionic strength: 50 mM; pH: 6.75; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1not availablesample_cond_1

Software:

FELIX v95.0 - processing

xwinnmr v2.1 - collection

ModelFree v4.01 - data analysis

NMR spectrometers:

  • BRUKER DMX 600 MHz
  • BRUKER DMX 750 MHz

Related Database Links:

BMRB 4527 4528 4762 4764 5303
PDB
DBJ BAJ39092 BAP09964
EMBL CAA59425 CAD03095 CAR35368 CAR39210 CAR61856
GB AAL22844 AAO71117 AAV79614 AAX67803 ABX70260
PIR AC0950
REF NP_458044 NP_462885 NP_807257 WP_001188773 WP_001188774
SP P41789
AlphaFold P41789