BMRB Entry 4720

Title:
Backbone 1H, 15N, and 13C Resonance Assignments of Inhibitor-2-- a Protein Inhibitor of Protein Phosphatase-1
Deposition date:
2000-04-13
Original release date:
2007-03-23
Authors:
Huang, Hsien-bin; Chen, Yi-Chen; Tsai, Li-huang; Wang, Hui-chun; Lin, Fang-Min; Horiuchi, Atsuko; Greengard, Paul; Nairn, Angus; Shiao, Ming-Shi; Lin, Ta-Hsien
Citation:

Citation: Huang, Hsien-bin; Chen, Yi-Chen; Tsai, Li-huang; Wang, Hui-chun; Lin, Fang-Min; Horiuchi, Atsuko; Greengard, Paul; Nairn, Angus; Shiao, Ming-Shi; Lin, Ta-Hsien. "Backbone 1H, 15N, and 13C Resonance Assignments of Inhibitor-2-- a Protein Inhibitor of Protein Phosphatase-1"  J. Biomol. NMR 17, 359-360 (2000).

Assembly members:

Assembly members:
Inhibitor-2, polymer, 173 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-3a

Data sets:
Data typeCount
13C chemical shifts509
15N chemical shifts162
1H chemical shifts641

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1inhibitor-21

Entities:

Entity 1, inhibitor-2 173 residues - Formula weight is not available

1   METALAALASERTHRALASERHISARGPRO
2   ILELYSGLYILELEULYSASNLYSTHRSER
3   THRTHRSERSERMETVALALASERALAGLU
4   GLNPROARGGLYASNVALASPGLUGLULEU
5   SERLYSLYSSERGLNLYSTRPASPGLUMET
6   ASNILELEUALATHRTYRHISPROALAASP
7   LYSASPTYRGLYLEUMETLYSILEASPGLU
8   PROSERTHRPROTYRHISSERMETMETGLY
9   ASPASPGLUASPALACYSSERASPTHRGLU
10   ALATHRGLUALAMETALAPROASPILELEU
11   ALAARGLYSLEUALAALAALAGLUGLYLEU
12   GLUPROLYSTYRARGILEGLNGLUGLNGLU
13   SERSERGLYGLUGLUASPSERASPLEUSER
14   PROGLUGLUARGGLULYSLYSARGGLNPHE
15   GLUMETLYSARGLYSLEUHISTYRASNGLU
16   GLYLEUASNILELYSLEUALAARGGLNLEU
17   ILESERLYSASPLEUHISASPASPASPGLU
18   ASPGLUGLU

Samples:

sample-1: Inhibitor-2, [U-13C; U-15N], 1.6 mM

exp1: pH: 6.0; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

BMRB 15179
DBJ BAI46858
EMBL CAA55475 CAA82754 CAB41680
GB AAC51206 AAH07655 AAL48322 ABW23430 ADQ32784
PRF 2007253A
REF NP_001247610 NP_001278433 NP_001303254 NP_006232 XP_002814482
SP P41236
AlphaFold P41236

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks