BMRB Entry 4454

Title:
1H and 15N Chemical Shift Assignments for Titin Z-repeat 7 in the Complex with Alpha-actinin C-terminal EF-hands
Deposition date:
1999-11-10
Original release date:
2000-03-22
Authors:
Atkinson, R.; Joseph, Catherine; Dal Piaz, Fabrizio; Birolo, Leyla; Stier, Gunter; Pucci, Piero; Pastore, Annalisa
Citation:

Citation: Atkinson, R.; Joseph, Catherine; Dal Piaz, Fabrizio; Birolo, Leyla; Stier, Gunter; Pucci, Piero; Pastore, Annalisa. "The Binding of Alpha-actinin to Titin: Implications for Z-disk Assembly"  Biochemistry ., .-..

Assembly members:

Assembly members:
alpha-actinin C-terminal EF-hands, polymer, 75 residues, 8071.05 Da.
titin Z-repeat 7, polymer, 51 residues, 5659.33 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts174
15N chemical shifts31
coupling constants31

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha-actinin_C-terminal EF-hands1
2titin Z-repeat 72

Entities:

Entity 1, alpha-actinin_C-terminal EF-hands 75 residues - 8071.05 Da.

1   GLYALAMETALAASPTHRASPTHRALAGLU
2   GLNVALILEALASERPHEARGILELEUALA
3   SERASPLYSPROTYRILELEUALAGLUGLU
4   LEUARGARGGLULEUPROPROASPGLNALA
5   GLNTYRCYSILELYSARGMETPROALATYR
6   SERGLYPROGLYSERVALPROGLYALALEU
7   ASPTYRALAALAPHESERSERALALEUTYR
8   GLYGLUSERASPLEU

Entity 2, titin Z-repeat 7 51 residues - 5659.33 Da.

1   METGLYLYSVALGLYVALGLYLYSLYSALA
2   GLUALAVALALATHRVALVALALAALAVAL
3   ASPGLNALAARGVALARGGLUPROARGGLU
4   PROGLYLEUPROGLUASPSERTYRALAGLN
5   GLNTHRTHRLEUGLUTYRGLYTYRLYSGLU
6   HIS

Samples:

sample_one: alpha-actinin C-terminal EF-hands 0.7 mM; titin Z-repeat 7, [U-15N], 0.7 mM; sodium phosphate 20.0 mM

sample_conditions: pH: 6.6 na; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_onenot availablesample_conditions
2D TOCSYsample_onenot availablesample_conditions
2D 1H-15N HSQCsample_onenot availablesample_conditions
3D 15N NOESY-HSQCsample_onenot availablesample_conditions
3D 15N TOCSY-HSQCsample_onenot availablesample_conditions
3D HNHAsample_onenot availablesample_conditions
3D HNHBsample_onenot availablesample_conditions

Software:

nmrPipe - processing

XEASY - analysis

NMR spectrometers:

  • Varian UnityPlus 500 MHz
  • Varian UnityPlus 600 MHz

Related Database Links:

BMRB 17626 17627 4453 4453
PDB
DBJ BAB22865 BAD92758 BAG37672 BAH11921 BAH12587
EMBL CAB61269 CAA77028
GB AAA51583 AAF76325 AAH47901 AAH51770 AAH89579
REF NP_001029807 NP_001094 NP_001163796 NP_001230595 NP_001265272
SP P35609 Q3ZC55 Q9JI91
AlphaFold P35609 Q3ZC55 Q9JI91

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks