BMRB Entry 4435

Title:
Structural Basis for the Network of Functional Cooperativities in Cytochrome c(3) from Desulfovibrio gigas: Solution Structures of the Oxidised and Reduced states
Deposition date:
1999-10-12
Original release date:
2000-12-07
Authors:
Brennan, L.; Turner, D.; Messias, A.; Teodoro, M.; Legall, J.; Santos, H.; Xavier, A.
Citation:

Citation: Brennan, L.; Turner, D.; Messias, A.; Teodoro, M.; Legall, J.; Santos, H.; Xavier, A.. "Structural Basis for the Network of Functional Cooperativities in Cytochrome c(3) from Desulfovibrio gigas: Solution Structures of the Oxidised and Reduced states"  J. Mol. Biol. 298, 61-82 (2000).

Assembly members:

Assembly members:
CYTOCHROME C3, polymer, 112 residues, 14000 Da.
HEC, non-polymer, 618.503 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 879   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Desulfovibrio gigas

Experimental source:

Experimental source:   Production method: purified from the natural source

Data typeCount
1H chemical shifts777

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cytochrome c31
2heme I2
3heme II2
4heme III2
5heme IV2

Entities:

Entity 1, cytochrome c3 112 residues - 14000 Da.

1   VALASPVALPROALAASPGLYALALYSILE
2   ASPPHEILEALAGLYGLYGLULYSASNLEU
3   THRVALVALPHEASNHISSERTHRHISLYS
4   ASPVALLYSCYSASPASPCYSHISHISGLN
5   PROGLYASPLYSGLNTYRALAGLYCYSTHR
6   THRASPGLYCYSHISASNILELEUASPLYS
7   ALAASPLYSSERVALASNSERTRPTYRLYS
8   VALVALHISASPALALYSGLYGLYALALYS
9   PROTHRCYSILESERCYSHISLYSASPLYS
10   ALAGLYASPASPLYSGLULEULYSLYSLYS
11   LEUTHRGLYCYSLYSGLYSERALACYSHIS
12   PROSER

Entity 2, heme I - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

sample_one: CYTOCHROME C3 2 mM; ampicillin 70 uM; kanamicine 50 uM; chloramphenicol 50 uM

sample_conditions: pH: 5.1 na; temperature: 306.4 K

Experiments:

NameSampleSample stateSample conditions
2D-1H-NOESYsample_onenot availablesample_conditions
2D-1H-TOCSYsample_onenot availablesample_conditions
2D-1H-COSYsample_onenot availablesample_conditions

Software:

XEASY -

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

BMRB 4436
PDB
EMBL CAB59196
GB AGW12081
REF WP_021758659
SP P00133
AlphaFold P00133