BMRB Entry 4404

Title:
Solution Structure of the Major alpha-amylase Inhibitor of the crop plant Amaranth
Deposition date:
1999-09-15
Original release date:
2000-12-07
Authors:
Lu, Shanyun; Deng, Pengchi; Liu, Xiucai; Luo, Jingchu; Han, Rushan; Gu, Xiaocheng; Liang, Songping; Wang, Xianchun; Li, Feng; Lozanov, Valentin; Patthy, Andras; Pongor, Sandor
Citation:

Citation: Lu, Shanyun; Deng, Pengchi; Liu, Xiucai; Luo, Jingchu; Han, Rushan; Gu, Xiaocheng; Liang, Songping; Wang, Xianchun; Li, Feng; Lozanov, Valentin; Patthy, Andras; Pongor, Sandor. "Solution Structure of the Major alpha-amylase Inhibitor of the crop plant Amaranth"  J. Biol. Chem. 274, 20473-20478 (1999).

Assembly members:

Assembly members:
alpha-amylase inhibitor, polymer, 32 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: grain amaranth   Taxonomy ID: 28502   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Amaranthus hypochondriacus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
alpha-amylase inhibitor: CIPKWNRCGPKMDGVPCCEP YTCTSDYYGNCS

Data sets:
Data typeCount
1H chemical shifts173
coupling constants8

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AAI monomer1

Entities:

Entity 1, AAI monomer 32 residues - Formula weight is not available

1   CYSILEPROLYSTRPASNARGCYSGLYPRO
2   LYSMETASPGLYVALPROCYSCYSGLUPRO
3   TYRTHRCYSTHRSERASPTYRTYRGLYASN
4   CYSSER

Samples:

sample_1: alpha-amylase inhibitor 5.0 mM

sample_2: alpha-amylase inhibitor 2.0 mM

Ex-cond_1: pH: 6.5; temperature: 300 K

Ex-cond_2: pH*: 6.5; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
1H-COSYnot availablenot availablenot available
1H-TOCSYnot availablenot availablenot available
1H-NOESYnot availablenot availablenot available

Software:

SYBYL v6.3 -

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

BMRB 4490
PDB
SP P80403
AlphaFold P80403