BMRB Entry 4158

Title:
1H and 15N Nuclear Magnetic Resonance Chemical Shifts of the Cytotoxic Ribonuclease Alpha-sarcin
Deposition date:
1998-07-03
Original release date:
2000-03-08
Authors:
Rico, Manuel; Bruix, Marta; Santoro, Jorge; Campos-Olivas, Ramon; Perez-Canadillas, Jose
Citation:

Citation: Campos-Olivas, Ramon; Bruix, Marta; Santoro, Jorge; Martinez del Pozo, Alvaro; Lacadena, Javier; Gavilanes, Jose; Rico, Manuel. "1H and 15N Nuclear Magnetic Resonance Assignment and Secondary Structure of the Cytotoxic Ribonuclease Alpha-sarcin"  Protein Sci. 5, 969-972 (1996).

Assembly members:

Assembly members:
Ribonuclease alpha-sarcin, polymer, 150 residues, 16981 Da.

Natural source:

Natural source:   Common Name: Aspergillus giganteus   Taxonomy ID: 5060   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Aspergillus giganteus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pINPGaS

Data sets:
Data typeCount
15N chemical shifts154
1H chemical shifts908

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha sarcin1

Entities:

Entity 1, alpha sarcin 150 residues - 16981 Da.

1   ALAVALTHRTRPTHRCYSLEUASNASPGLN
2   LYSASNPROLYSTHRASNLYSTYRGLUTHR
3   LYSARGLEULEUTYRASNGLNASNLYSALA
4   GLUSERASNSERHISHISALAPROLEUSER
5   ASPGLYLYSTHRGLYSERSERTYRPROHIS
6   TRPPHETHRASNGLYTYRASPGLYASPGLY
7   LYSLEUPROLYSGLYARGTHRPROILELYS
8   PHEGLYLYSSERASPCYSASPARGPROPRO
9   LYSHISSERLYSASPGLYASNGLYLYSTHR
10   ASPHISTYRLEULEUGLUPHEPROTHRPHE
11   PROASPGLYHISASPTYRLYSPHEASPSER
12   LYSLYSPROLYSGLUASNPROGLYPROALA
13   ARGVALILETYRTHRTYRPROASNLYSVAL
14   PHECYSGLYILEILEALAHISTHRLYSGLU
15   ASNGLNGLYGLULEULYSLEUCYSSERHIS

Samples:

sample_one: Ribonuclease alpha-sarcin, [U-15N], 1.5 mM

sample_conditions_one: pH: 4.0; temperature: 306 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB
DBJ BAA02863
EMBL CAA37471 CAA43180
GB AAB66603 AAB66605 ACA34736
SP P00655
AlphaFold P00655

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks