BMRB Entry 4075

Title:
Backbone NMR Assignments and Secondary Structure of the N Terminal Domain of DnaB Helicase from E. coli
Deposition date:
1997-11-25
Original release date:
1998-10-12
Authors:
Weigelt, Johan; Miles, Caroline; Dixon, Nicholas; Otting, Gottfried
Citation:

Citation: Weigelt, Johan; Miles, Caroline; Dixon, Nicholas; Otting, Gottfried. "Backbone NMR Assignments and Secondary Structure of the N Terminal Domain of DnaB Helicase from E. coli"  J. Biomol. NMR 11, 233-234 (1998).

Assembly members:

Assembly members:
DnaB helicase, polymer, 142 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pCM865

Data sets:
Data typeCount
13C chemical shifts253
15N chemical shifts124
1H chemical shifts259

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DnaB-Nterm1

Entities:

Entity 1, DnaB-Nterm 142 residues - Formula weight is not available

1   ALAGLYASNLYSPROPHEASNLYSGLNGLN
2   ALAGLUPROARGGLUARGASPPROGLNVAL
3   ALAGLYLEULYSVALPROPROHISSERILE
4   GLUALAGLUGLNSERVALLEUGLYGLYLEU
5   METLEUASPASNGLUARGTRPASPASPVAL
6   ALAGLUARGVALVALALAASPASPPHETYR
7   THRARGPROHISARGHISILEPHETHRGLU
8   METALAARGLEUGLNGLUSERGLYSERPRO
9   ILEASPLEUILETHRLEUALAGLUSERLEU
10   GLUARGGLNGLYGLNLEUASPSERVALGLY
11   GLYPHEALATYRLEUALAGLULEUSERLYS
12   ASNTHRPROSERALAALAASNILESERALA
13   TYRALAASPILEVALARGGLUARGALAVAL
14   VALARGGLUMETILESERVALALAASNGLU
15   ILEALA

Samples:

sample_one: DnaB helicase, [U-13C;U-15N], 1.8 mM; sodium phosphate 20 mM; pefabloc 1 mM; EDTA 1 mM; NaN3 0.02 % w/v

sample_conditions: pH: 6.5; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 4297
PDB
DBJ BAB38457 BAE78054 BAG66812 BAG79869 BAH61192
EMBL CAP78509 CAQ34401 CAQ91563 CAR01031 CAR05689
GB AAA23689 AAA23690 AAC43146 AAC77022 AAG59250
REF NP_313061 NP_418476 NP_709868 WP_000918354 WP_000918355
SP P0ACB0 P0ACB1 Q8FB22 Q8X5V3
AlphaFold P0ACB0 P0ACB1 Q8FB22 Q8X5V3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks