BMRB Entry 36220

Name: Solution structure of the N-terminal domain of the anti-sigma factor RsgI1 from Clostridium thermocellum
Published: 2020-09-19

Click here to enlarge.

PDB ID: 6ivs
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36220
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structure of the N-terminal domain of the anti-sigma factor RsgI1 from Clostridium thermocellum

Deposition date:

2018-12-04

Original release date:

2020-09-19

Authors:

Wei, Z.; Feng, Y.

Citation:

Citation: Wei, Z.; Chen, C.; Liu, Y.; Dong, S.; Li, J.; Qi, K.; Liu, S.; Ding, X.; Ora, L.; Munoz-Gutierrez, I.; Li, Y.; Yao, H.; Lamed, R.; Bayer, E.; Cui, Q.; Feng, Y.. "Alternative sigma I/anti-sigma I factors represent a unique form of bacterial sigma/anti-sigma complex" Nucleic Acids Res. 47, 5988-5997 (2019).
PubMed: 31106374

Assembly members:

Assembly members:
entity_1, polymer, 53 residues, 6170.172 Da.

Natural source:

Natural source: Common Name: Ruminiclostridium thermocellum Taxonomy ID: 203119 Superkingdom: Bacteria Kingdom: not available Genus/species: Hungateiclostridium Hungateiclostridium thermocellum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SMNRLGIIYEIQGMKAVVLT SEGEFLIIRRRKDMKVGQQV SFENEDIYNVRGK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	243
15N chemical shifts	62
1H chemical shifts	403

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 53 residues - 6170.172 Da.

1

SER

MET

ASN

ARG

LEU

GLY

ILE

TYR

GLU

2

ILE

GLN

GLY

MET

LYS

ALA

VAL

LEU

THR

3

SER

GLU

GLY

GLU

PHE

LEU

ILE

ARG

4

ARG

LYS

ASP

MET

LYS

VAL

GLY

GLN

VAL

5

SER

PHE

GLU

ASN

GLU

ASP

ILE

TYR

ASN

VAL

6

ARG

GLY

LYS

Samples:

sample_1: RsgI1 N-terminal domain, [U-13C; U-15N], 1.0 mM; Bis-Tris 20 mM; EDTA 2 mM; sodium chloride 50 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 50 mM; pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHANH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
3D CCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D CCH-COSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMR spectrometers:

Bruker AvanceIII 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks