BMRB Entry 36175

Name: A-ubiquitin like protein from the trypanosoma brucei
Published: 2018-12-07

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PDB ID: 5zmb
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR36175
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

A-ubiquitin like protein from the trypanosoma brucei

Deposition date:

2018-04-02

Original release date:

2018-12-07

Authors:

Mi, J.; Tu, X.

Citation:

Citation: Mi, J.; Zhang, J.; Liao, S.; Tu, X.. "Solution structure of a ubiquitin-like protein from Trypanosoma brucei" Protein Sci. 27, 1831-1836 (2018).
PubMed: 30058168

Assembly members:

Assembly members:
ubiquintin-like protein, polymer, 81 residues, 9141.467 Da.

Natural source:

Natural source: Common Name: Trypanosoma brucei Taxonomy ID: 185431 Superkingdom: Metazoa Kingdom: not available Genus/species: Trypanosoma brucei

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ubiquintin-like protein: MSETIPVSVQCCEGRFELSV DSNHTLRDVLRQFKREVAAL DPINLEEYVVNHEGKLLLDD SVTLQTVGVKKDSVFVLVRK A

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	236
15N chemical shifts	81
1H chemical shifts	497

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 81 residues - 9141.467 Da.

1

MET

SER

GLU

THR

ILE

PRO

VAL

SER

VAL

GLN

2

CYS

GLU

GLY

ARG

PHE

GLU

LEU

SER

VAL

3

ASP

SER

ASN

HIS

THR

LEU

ARG

ASP

VAL

LEU

4

ARG

GLN

PHE

LYS

ARG

GLU

VAL

ALA

LEU

5

ASP

PRO

ILE

ASN

LEU

GLU

TYR

VAL

6

ASN

HIS

GLU

GLY

LYS

LEU

ASP

7

SER

VAL

THR

LEU

GLN

THR

VAL

GLY

VAL

LYS

8

LYS

ASP

SER

VAL

PHE

VAL

LEU

VAL

ARG

LYS

9

ALA

Samples:

sample_1: ubiquitin-like protein, [U-99% 13C; U-99% 15N], 0.5 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 120 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure calculation

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

Bruker DMX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks